Detailed information of TA system TA214433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 4586975..4588168
• Replicon: chromosome
• Accession: NZ_CP082236
• Organism: Pseudomonas sp. SC3(2021)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K5Y35_RS20500
• Protein ID: WP_222832773.1
• Coordinates: 4586975..4587547 (-)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K5Y35_RS20505
• Protein ID: WP_261991204.1
• Coordinates: 4587623..4588168 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5Y35_RS20480	4583519..4584286	-	768	WP_123464304.1	phosphonate ABC transporter, permease protein PhnE	-
K5Y35_RS20485	4584283..4585107	-	825	WP_222832770.1	ABC transporter permease subunit	-
K5Y35_RS20490	4585101..4585898	-	798	WP_222832771.1	ATP-binding cassette domain-containing protein	-
K5Y35_RS20495	4585895..4586758	-	864	WP_222832772.1	putative selenate ABC transporter substrate-binding protein	-
K5Y35_RS20500	4586975..4587547	-	573	WP_222832773.1	HD domain-containing protein	Toxin
K5Y35_RS20505	4587623..4588168	-	546	WP_261991204.1	helix-turn-helix transcriptional regulator	Antitoxin
K5Y35_RS20510	4588254..4588655	-	402	WP_123464315.1	RidA family protein	-
K5Y35_RS20515	4588771..4589664	+	894	WP_123464320.1	LysR family transcriptional regulator	-
K5Y35_RS20520	4589675..4590007	+	333	WP_261991205.1	hypothetical protein	-
K5Y35_RS20525	4589978..4590865	-	888	WP_123464322.1	LysR substrate-binding domain-containing protein	-
K5Y35_RS20530	4590977..4592029	+	1053	WP_222832775.1	agmatine deiminase family protein	-
K5Y35_RS20535	4592033..4592941	+	909	WP_222832776.1	N-carbamoylputrescine amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-171)

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-42)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21380.24 Da
• Isoelectric Point: 5.5921

>T214433 WP_222832773.1 NZ_CP082236:c4587547-4586975 [Pseudomonas sp. SC3(2021)]
MDLEKISGRLEFLREAEKLKDVLRSAHTSSGRTESTAEHSWRLCLMAIVFADELAGLDLLKVLRMCVIHDLGEAINGDIP
AVDQAAFPDKSEQERNDLLLLTRSLDNTLRNEILALWDDYENARSAEAKAVKALDKLETLLQHTQGHNPADFDYRFNLTY
GKKHTSAAPIFAALRALIDQDTHARIQQAK

Nucleotide

• Length: 573 bp

>T214433 NZ_CP110072:2803499-2803594 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19952.05 Da
• Isoelectric Point: 7.7601

>AT214433 WP_261991204.1 NZ_CP082236:c4588168-4587623 [Pseudomonas sp. SC3(2021)]
MLKPALPGIALRRWRLLHRVKQSHAAQMFSVTQSTISRWESGVLALDPAAHLQLEQLLAARLDTAADQALARLVCESARP
VHLVCDLTHRLLACSPARAAQFSSPLSELLGQSLWPFVTPEIANNERALDTLGWRELPAPPALEFVTGNNDSDLVPIRQS
LCRWTRIPLSDGTAARLVETL

Nucleotide

• Length: 546 bp

>AT214433 NZ_CP110072:c2803694-2803629 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATCAGTAGTAGGGTGTCTTTTTTT