Detailed information of TA system TA214431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1771616..1773243
• Replicon: chromosome
• Accession: NZ_CP082236
• Organism: Pseudomonas sp. SC3(2021)

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: K5Y35_RS07760
• Protein ID: WP_128613605.1
• Coordinates: 1771616..1772968 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: K5Y35_RS07765
• Protein ID: WP_128613606.1
• Coordinates: 1772965..1773243 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5Y35_RS07755	1768429..1771386	-	2958	WP_222834019.1	autotransporter outer membrane beta-barrel domain-containing protein	-
K5Y35_RS07760	1771616..1772968	+	1353	WP_128613605.1	HipA domain-containing protein	Toxin
K5Y35_RS07765	1772965..1773243	+	279	WP_128613606.1	helix-turn-helix transcriptional regulator	Antitoxin
K5Y35_RS07770	1773276..1774112	-	837	WP_222834020.1	amino acid ABC transporter permease	-
K5Y35_RS07775	1774109..1774930	-	822	WP_222834021.1	ABC transporter substrate-binding protein	-
K5Y35_RS07780	1775044..1775943	+	900	WP_128613608.1	homocysteine S-methyltransferase family protein	-
K5Y35_RS07785	1776032..1776703	-	672	WP_222834022.1	GNAT family protein	-
K5Y35_RS07790	1776716..1777162	-	447	WP_123469978.1	GNAT family N-acetyltransferase	-
K5Y35_RS07795	1777173..1777796	-	624	WP_222834023.1	FMN-binding negative transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-367)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-80)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 50273.17 Da
• Isoelectric Point: 6.0808

>T214431 WP_128613605.1 NZ_CP082236:1771616-1772968 [Pseudomonas sp. SC3(2021)]
MYNLTLQIFTAGKWQDAMILSFDDPEKGFESRCSFGYETAYLAENIEAMGSPFSRAVSALYPLDWDGHRSKTPAFVHDIA
PAGAAKRFLLERLGREKPANISADLYLLGRSTPAPIGNMRIKESAEAVDERKPIGFKREDVVHRDNRFLEYAYELGAAIG
GATGAGGEAPKLLLAENKAGLLYPDAVLDDDKVRQHWFVKFARNRGGLTDQDILRSEFHYYKALQKLGIETIAAEGLALE
EANKPSLWMQRFDRKVRDGDVSRLAVESIYSLAQVTTPGSAMNHMEVIRMLAGLWRQAGQASQIPALVTDYLRRDLINKI
LGNSDNHGRNTAIIRQANTFRLAPIYDLAPMVMDDEGVTRTTKWPRDLERAGDVDWRGVCRALADIAEPNDPLAALCDAP
ALFERLREDAQRLAALPDILTASGLPDRTMNHPGIHLKNLEQRLKEWDLQ

Nucleotide

• Length: 1353 bp

>T214431 NZ_CP110072:c2488738-2488622 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10343.14 Da
• Isoelectric Point: 10.7364

>AT214431 WP_128613606.1 NZ_CP082236:1772965-1773243 [Pseudomonas sp. SC3(2021)]
MTMTVAERTVLIESIQEALAQGTLEIGEAVRRLRVEVTGLHQTQFAKMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGL
KMGVVRVRREIN

Nucleotide

• Length: 279 bp

>AT214431 NZ_CP110072:2488506-2488711 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC