Detailed information of TA system TA214429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 975229..975777
• Replicon: chromosome
• Accession: NZ_CP082236
• Organism: Pseudomonas sp. SC3(2021)

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K5Y35_RS04390
• Protein ID: WP_222833758.1
• Coordinates: 975229..975483 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W6VRS3
• Locus tag: K5Y35_RS04395
• Protein ID: WP_003221244.1
• Coordinates: 975487..975777 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5Y35_RS04370	970566..971954	-	1389	WP_222833755.1	precorrin-3B synthase	-
K5Y35_RS04375	971997..973208	+	1212	WP_123471024.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
K5Y35_RS04380	973201..974298	+	1098	WP_222833756.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
K5Y35_RS04385	974295..975023	+	729	WP_222833757.1	cobalt-precorrin-6A reductase	-
K5Y35_RS04390	975229..975483	+	255	WP_222833758.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5Y35_RS04395	975487..975777	+	291	WP_003221244.1	putative addiction module antidote protein	Antitoxin
K5Y35_RS04400	975876..976274	+	399	WP_123471018.1	DUF2946 domain-containing protein	-
K5Y35_RS04405	976323..976799	+	477	WP_123471016.1	copper chaperone PCu(A)C	-
K5Y35_RS04410	976821..977255	+	435	WP_222833759.1	DUF2946 domain-containing protein	-
K5Y35_RS04415	977341..979461	+	2121	WP_222833760.1	TonB-dependent copper receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-79)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9606.95 Da
• Isoelectric Point: 9.7861

>T214429 WP_222833758.1 NZ_CP082236:975229-975483 [Pseudomonas sp. SC3(2021)]
MKDIIGKGRIISRLRAAEHGNFGDYGFVGDTVYEMRVHYGPGYRMSFTRRDDVIYLLLIGGDKSTQRRDIKRAVQMAHSI
GNEE

Nucleotide

• Length: 255 bp

>T214429 NZ_CP110072:c1241239-1240895 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTCGCTCCAATAAATAAGCGCGCATGTTACGA
GCCAGTCAAAAGAATAATTTTCGTAAATCAAAATTTATCTATCGAAGAACAAGAAGAGTCTATATTCCATGAGTTCAAAC
ATGTTGTCTCCCATTCGGATTATATTGAGTTATATAAAATTCCTTCTTTTAGAAATAAGATGGAAGCTGAGGCAGATTAT
CATATGTTTAAATGTCTTATTGAAAAACATGACGGGCAATTTAATTATTCTAATGTGATTACTCATTACAATTTAAAGAT
GGGACAGGAAACTTATTTAAATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10430.91 Da
• Isoelectric Point: 4.6027

>AT214429 WP_003221244.1 NZ_CP082236:975487-975777 [Pseudomonas sp. SC3(2021)]
MTEKFTRFDAAEYLKTPEDMAAYLDACFDDDEGDGVLIRAALNDIARAQGMTQVARDAGLGRESLYKALSSTGNPEFATI
MKVMKALGLRLHAVAV

Nucleotide

• Length: 291 bp

>AT214429 NZ_CP110072:c1241588-1241256 [Enterococcus faecalis]
ATGAACCTCTTGGAACGTATAAAAAAACTAGCAAAAGATAGAGATGTGTCTATTTATCAGCTTGAAGAAGAAATAAATAT
TGGACGAAATACTATTTATCAATGGAATAAGCGAACACCTTCTTCGGATAAACTTGAAGCTGTAGCCAATTATTTTAATG
TTTCTGTTGATTATTTATTAGGTCGTACTGAAAATCCAAACCAAGCTGGTGCTAAACCATCTGATGATTTAGATGAAGTA
TTAGACAATGTGATGAGTTTCGATGGTGAGCCTTTGGATGATCATGATCGGGAAGTTATTCGTGCTTATTTGAAAGGGAG
ATTCGGAAAATAA

Structures

Antitoxin

• AlphaFold DB: W6VRS3