Detailed information of TA system TA214427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 54776..55347
• Replicon: plasmid pE006-101
• Accession: NZ_CP082232
• Organism: Enterococcus faecalis strain E006

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A2Z2CGM4
• Locus tag: K4200_RS14120
• Protein ID: WP_002362432.1
• Coordinates: 55006..55347 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3KHK9
• Locus tag: K4200_RS14115
• Protein ID: WP_002362431.1
• Coordinates: 54776..55006 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4200_RS14075 (50720)	50720..51019	-	300	WP_010817802.1	hypothetical protein	-
- (51128)	51128..51219	+	92	NuclAT_0	-	-
- (51128)	51128..51219	+	92	NuclAT_0	-	-
- (51128)	51128..51219	+	92	NuclAT_0	-	-
- (51128)	51128..51219	+	92	NuclAT_0	-	-
K4200_RS14080 (51259)	51259..51369	-	111	WP_002360667.1	type I toxin-antitoxin system Fst family toxin	-
K4200_RS14085 (51864)	51864..52181	+	318	WP_002394802.1	heavy metal-binding domain-containing protein	-
K4200_RS14090 (52217)	52217..52885	+	669	WP_002403283.1	CPBP family intramembrane metalloprotease	-
K4200_RS14095 (53002)	53002..53256	+	255	WP_002394800.1	hypothetical protein	-
K4200_RS14100 (53416)	53416..53649	-	234	WP_002394799.1	hypothetical protein	-
K4200_RS14105 (53651)	53651..53935	-	285	WP_002394798.1	hypothetical protein	-
K4200_RS14110 (53952)	53952..54572	-	621	WP_162500025.1	recombinase family protein	-
K4200_RS14115 (54776)	54776..55006	+	231	WP_002362431.1	hypothetical protein	Antitoxin
K4200_RS14120 (55006)	55006..55347	+	342	WP_002362432.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K4200_RS14125 (55459)	55459..56397	+	939	WP_002362433.1	hypothetical protein	-
K4200_RS14130 (56662)	56662..57264	+	603	WP_010711821.1	Fic family protein	-
K4200_RS14135 (57513)	57513..59351	+	1839	WP_010711822.1	P-loop NTPase fold protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / dfrG / lnu(G) / erm(A) / optrA / ant(6)-Ia	prgB/asc10	1..101692	101692

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13244.44 Da
• Isoelectric Point: 8.0113

>T214427 WP_002362432.1 NZ_CP082232:55006-55347 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T214427 NZ_CP110072:265144-265485 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8510.42 Da
• Isoelectric Point: 4.3855

>AT214427 WP_002362431.1 NZ_CP082232:54776-55006 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT214427 NZ_CP110072:264914-265144 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A2Z2CGM4

Antitoxin

• AlphaFold DB: R3KHK9