214419

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2734321..2734892 Replicon chromosome
Accession NZ_CP082231
Organism Enterococcus faecalis strain E006

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag K4200_RS13340 Protein ID WP_002360937.1
Coordinates 2734321..2734662 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3JGB1
Locus tag K4200_RS13345 Protein ID WP_002354773.1
Coordinates 2734662..2734892 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K4200_RS13335 (2730336) 2730336..2733950 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
K4200_RS13340 (2734321) 2734321..2734662 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K4200_RS13345 (2734662) 2734662..2734892 - 231 WP_002354773.1 hypothetical protein Antitoxin
K4200_RS13350 (2735299) 2735299..2735514 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
K4200_RS13355 (2735653) 2735653..2736645 + 993 WP_023894581.1 biotin--[acetyl-CoA-carboxylase] ligase -
K4200_RS13360 (2736712) 2736712..2737344 - 633 WP_002358972.1 RloB family protein -
K4200_RS13365 (2737353) 2737353..2738648 - 1296 WP_010820355.1 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T214419 WP_002360937.1 NZ_CP082231:c2734662-2734321 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T214419 NZ_CP110071:265144-265485 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.67 Da        Isoelectric Point: 4.3313

>AT214419 WP_002354773.1 NZ_CP082231:c2734892-2734662 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT214419 NZ_CP110071:264914-265144 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2A812

References