Detailed information of TA system TA214404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 2378933..2379628
• Replicon: chromosome
• Accession: NZ_CP082231
• Organism: Enterococcus faecalis strain E006

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: K4200_RS11700
• Protein ID: WP_002385632.1
• Coordinates: 2379284..2379628 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: K4200_RS11695
• Protein ID: WP_002385633.1
• Coordinates: 2378933..2379265 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4200_RS11645 (2374295)	2374295..2375110	-	816	WP_002393795.1	PD-(D/E)XK nuclease-like domain-containing protein	-
K4200_RS11650 (2375073)	2375073..2376089	-	1017	WP_002393794.1	RecT family recombinase	-
K4200_RS11655 (2376192)	2376192..2376416	-	225	WP_002370012.1	hypothetical protein	-
K4200_RS11660 (2376413)	2376413..2376712	-	300	WP_002385643.1	hypothetical protein	-
K4200_RS11665 (2376779)	2376779..2376958	-	180	WP_010785685.1	hypothetical protein	-
K4200_RS11670 (2376994)	2376994..2377263	-	270	WP_002385641.1	hypothetical protein	-
K4200_RS11675 (2377339)	2377339..2377653	+	315	WP_002385638.1	hypothetical protein	-
K4200_RS14580 (2377634)	2377634..2377762	-	129	WP_002385637.1	hypothetical protein	-
K4200_RS11680 (2377778)	2377778..2377954	-	177	WP_002385636.1	helix-turn-helix transcriptional regulator	-
K4200_RS11685 (2378038)	2378038..2378421	+	384	WP_159110597.1	DUF2513 domain-containing protein	-
K4200_RS11690 (2378418)	2378418..2378627	-	210	WP_002415277.1	DUF2829 domain-containing protein	-
K4200_RS11695 (2378933)	2378933..2379265	+	333	WP_002385633.1	helix-turn-helix transcriptional regulator	Antitoxin
K4200_RS11700 (2379284)	2379284..2379628	+	345	WP_002385632.1	ImmA/IrrE family metallo-endopeptidase	Toxin
K4200_RS11705 (2379686)	2379686..2380414	+	729	WP_002385631.1	potassium channel family protein	-
K4200_RS11710 (2380569)	2380569..2381750	+	1182	WP_010714109.1	site-specific integrase	-
K4200_RS11715 (2381853)	2381853..2382002	-	150	WP_002356321.1	50S ribosomal protein L33	-
K4200_RS11720 (2382128)	2382128..2384263	-	2136	WP_165548655.1	penicillin-binding protein 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13594.61 Da
• Isoelectric Point: 5.6229

>T214404 WP_002385632.1 NZ_CP082231:2379284-2379628 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKEHVDIMALYKIPVFRSKMEAEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEVYLK

Nucleotide

• Length: 345 bp

>T214404 NZ_CP110068:c2813490-2813386 [Enterococcus faecalis]
TTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGGTATGTTTACCATTGCTTTGATCGCGCTGATTGTGAAATT
GCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12677.13 Da
• Isoelectric Point: 4.6211

>AT214404 WP_002385633.1 NZ_CP082231:2378933-2379265 [Enterococcus faecalis]
MNLLERIKKLAKDRDVSIYQLEEEINIGRNTIYQWNKRTPSSDKLEAVANYFNVSVDYLLGRTENPNQAGAKPSDDLDDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT214404 NZ_CP110068:2813267-2813453 [Enterococcus faecalis]
TGTGCTATAATGAAAATGAAAAGAGAGATATGCCTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCAATT
TAGTTATAAAAAATAACCGTACTCGATCAAAGTAAACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGCG
CGATCAAAGCAATGGTAAACATACCAA