Detailed information of TA system TA214386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 2223637..2224253
• Replicon: chromosome
• Accession: NZ_CP082217
• Organism: Xanthomonas cucurbitae strain KS_456

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A853FWE4
• Locus tag: K6980_RS09290
• Protein ID: WP_048279626.1
• Coordinates: 2223957..2224253 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K6980_RS09285
• Protein ID: WP_048279629.1
• Coordinates: 2223637..2223951 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6980_RS09265 (K6980_09225)	2220089..2221297	+	1209	WP_016941853.1	site-specific integrase	-
K6980_RS09270 (K6980_09230)	2221582..2221812	+	231	WP_104604568.1	AlpA family phage regulatory protein	-
K6980_RS09275 (K6980_09235)	2221816..2222697	+	882	WP_104604570.1	helicase RepA family protein	-
K6980_RS09280 (K6980_09240)	2222684..2223436	+	753	WP_104604572.1	ABC transporter ATPase	-
K6980_RS09285 (K6980_09245)	2223637..2223951	-	315	WP_048279629.1	putative addiction module antidote protein	Antitoxin
K6980_RS09290 (K6980_09250)	2223957..2224253	-	297	WP_048279626.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6980_RS09295 (K6980_09255)	2224699..2225025	-	327	WP_032422605.1	hypothetical protein	-
K6980_RS09300	2225028..2225435	-	408	WP_249027663.1	hypothetical protein	-
K6980_RS09305 (K6980_09265)	2225730..2227279	+	1550	Protein_1841	IS3 family transposase	-
K6980_RS09310 (K6980_09270)	2227374..2228207	-	834	Protein_1842	P-type conjugative transfer protein TrbL	-
K6980_RS09315 (K6980_09275)	2228312..2228494	-	183	WP_004690111.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2215156..2234569	19413

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-91)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11147.01 Da
• Isoelectric Point: 10.4422

>T214386 WP_048279626.1 NZ_CP082217:c2224253-2223957 [Xanthomonas cucurbitae]
MIELIKTDLFDRWLVGLRDRQARARIEARIRRLSLGNPGDVKPVGEGLSEMRIDHGPGYRVYFMRRGPLVVLLLCGGDKS
SQERDIALAKTIAAQWKE

Nucleotide

• Length: 297 bp

>T214386 NZ_CP110060:68697-69038 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAATATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 10802.21 Da
• Isoelectric Point: 4.7393

>AT214386 WP_048279629.1 NZ_CP082217:c2223951-2223637 [Xanthomonas cucurbitae]
MEVKFSRYDTADYLKTEDDIAAYLDAVMEDGDPALIAAALGDVARARNLSQLARDVGMSRQGLDKALSGDGNPSLATVVK
VAQALGLRLTFKPTHGGPAPAAGA

Nucleotide

• Length: 315 bp

>AT214386 NZ_CP110060:68467-68697 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTAATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Structures

Toxin

• AlphaFold DB: A0A853FWE4