Detailed information of TA system TA214371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 2271230..2271846
• Replicon: chromosome
• Accession: NZ_CP082213
• Organism: Xanthomonas cucurbitae strain WI_206

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A853FWE4
• Locus tag: K6981_RS10055
• Protein ID: WP_048279626.1
• Coordinates: 2271230..2271526 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K6981_RS10060
• Protein ID: WP_048279629.1
• Coordinates: 2271532..2271846 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6981_RS10025 (K6981_10015)	2266288..2266512	+	225	WP_104605634.1	hypothetical protein	-
K6981_RS10030 (K6981_10020)	2266782..2267573	+	792	WP_104605632.1	P-type conjugative transfer protein TrbJ	-
K6981_RS10035 (K6981_10025)	2267576..2267758	+	183	WP_004690111.1	hypothetical protein	-
K6981_RS10040 (K6981_10030)	2267761..2269386	+	1626	WP_274397155.1	P-type conjugative transfer protein TrbL	-
K6981_RS10045 (K6981_10035)	2269420..2270370	+	951	WP_159406945.1	IS30 family transposase	-
K6981_RS10050 (K6981_10040)	2270458..2270784	+	327	WP_032422605.1	hypothetical protein	-
K6981_RS10055 (K6981_10045)	2271230..2271526	+	297	WP_048279626.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6981_RS10060 (K6981_10050)	2271532..2271846	+	315	WP_048279629.1	putative addiction module antidote protein	Antitoxin
K6981_RS10065 (K6981_10055)	2272047..2272799	-	753	WP_104604572.1	ABC transporter ATPase	-
K6981_RS10070 (K6981_10060)	2272786..2273667	-	882	WP_104604570.1	helicase RepA family protein	-
K6981_RS10075 (K6981_10065)	2273671..2273901	-	231	WP_104604568.1	AlpA family phage regulatory protein	-
K6981_RS10080 (K6981_10070)	2274186..2275394	-	1209	WP_016941853.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2261594..2280327	18733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-91)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11147.01 Da
• Isoelectric Point: 10.4422

>T214371 WP_048279626.1 NZ_CP082213:2271230-2271526 [Xanthomonas cucurbitae]
MIELIKTDLFDRWLVGLRDRQARARIEARIRRLSLGNPGDVKPVGEGLSEMRIDHGPGYRVYFMRRGPLVVLLLCGGDKS
SQERDIALAKTIAAQWKE

Nucleotide

• Length: 297 bp

>T214371 NZ_CP110054:2759346-2759486 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAGAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 10802.21 Da
• Isoelectric Point: 4.7393

>AT214371 WP_048279629.1 NZ_CP082213:2271532-2271846 [Xanthomonas cucurbitae]
MEVKFSRYDTADYLKTEDDIAAYLDAVMEDGDPALIAAALGDVARARNLSQLARDVGMSRQGLDKALSGDGNPSLATVVK
VAQALGLRLTFKPTHGGPAPAAGA

Nucleotide

• Length: 315 bp

>AT214371 NZ_CP110054:c2759620-2759419 [Enterococcus faecalis]
GTTTTTTTAGGTGATTGTGCTGTAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGATCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A853FWE4