Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5338143..5338768 Replicon chromosome
Accession NZ_CP082212
Organism Klebsiella pneumoniae strain BJ20

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6039_RS26345 Protein ID WP_002882817.1
Coordinates 5338143..5338526 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6039_RS26350 Protein ID WP_004150355.1
Coordinates 5338526..5338768 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6039_RS26330 (K6039_26275) 5335509..5336411 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6039_RS26335 (K6039_26280) 5336408..5337043 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6039_RS26340 (K6039_26285) 5337040..5337969 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6039_RS26345 (K6039_26290) 5338143..5338526 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6039_RS26350 (K6039_26295) 5338526..5338768 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6039_RS26355 (K6039_26300) 5338973..5339890 + 918 WP_002882812.1 alpha/beta hydrolase -
K6039_RS26360 (K6039_26305) 5339904..5340845 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6039_RS26365 (K6039_26310) 5340890..5341327 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6039_RS26370 (K6039_26315) 5341324..5342184 - 861 WP_002882807.1 virulence factor BrkB family protein -
K6039_RS26375 (K6039_26320) 5342178..5342777 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T214368 WP_002882817.1 NZ_CP082212:c5338526-5338143 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T214368 NZ_CP110054:c2752684-2752343 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT214368 WP_004150355.1 NZ_CP082212:c5338768-5338526 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT214368 NZ_CP110054:c2752914-2752684 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGTATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References