Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4749844..4750654 Replicon chromosome
Accession NZ_CP082212
Organism Klebsiella pneumoniae strain BJ20

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag K6039_RS23520 Protein ID WP_002887280.1
Coordinates 4749844..4750377 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K6039_RS23525 Protein ID WP_002887278.1
Coordinates 4750388..4750654 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6039_RS23515 (K6039_23465) 4748675..4749796 + 1122 WP_002887282.1 cupin domain-containing protein -
K6039_RS23520 (K6039_23470) 4749844..4750377 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
K6039_RS23525 (K6039_23475) 4750388..4750654 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K6039_RS23530 (K6039_23480) 4750757..4752190 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
K6039_RS23535 (K6039_23485) 4752180..4752863 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K6039_RS23540 (K6039_23490) 4753035..4754420 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
K6039_RS23545 (K6039_23495) 4754438..4754782 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T214365 WP_002887280.1 NZ_CP082212:c4750377-4749844 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T214365 NZ_CP110054:514080-514196 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTAGTTTTGCAATACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT214365 WP_002887278.1 NZ_CP082212:c4750654-4750388 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT214365 NZ_CP110054:c514312-514107 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTATTGCAAAACTAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References