Detailed information of TA system TA021436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1511000..1511638
• Replicon: chromosome
• Accession: NC_007779
• Organism: Escherichia coli str. K-12 substr. W3110

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: Y75_RS07595
• Protein ID: WP_000813794.1
• Coordinates: 1511000..1511176 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: Y75_RS07600
• Protein ID: WP_001270286.1
• Coordinates: 1511222..1511638 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y75_RS07575 (Y75_p1409)	1506619..1507833	-	1215	WP_001326689.1	BenE family transporter YdcO	-
Y75_RS07580 (Y75_p1410)	1507886..1508422	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
Y75_RS07585 (Y75_p1411)	1508495..1510456	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
Y75_RS07590 (Y75_p1412)	1510548..1510778	-	231	WP_000494244.1	YncJ family protein	-
Y75_RS07595 (Y75_p1413)	1511000..1511176	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
Y75_RS07600 (Y75_p1414)	1511222..1511638	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
Y75_RS07605 (Y75_p1415)	1511717..1513123	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
Y75_RS07610 (Y75_p1416)	1513368..1514513	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
Y75_RS07615 (Y75_p1417)	1514531..1515544	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
Y75_RS07620 (Y75_p1418)	1515545..1516486	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T21436 WP_000813794.1 NC_007779:1511000-1511176 [Escherichia coli str. K-12 substr. W3110]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T21436 NC_007779:1511000-1511176 [Escherichia coli str. K-12 substr. W3110]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT21436 WP_001270286.1 NC_007779:1511222-1511638 [Escherichia coli str. K-12 substr. W3110]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT21436 NC_007779:1511222-1511638 [Escherichia coli str. K-12 substr. W3110]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB