Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5267357..5267982 Replicon chromosome
Accession NZ_CP082211
Organism Klebsiella pneumoniae strain A1718

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6036_RS25300 Protein ID WP_002882817.1
Coordinates 5267357..5267740 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6036_RS25305 Protein ID WP_004150355.1
Coordinates 5267740..5267982 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6036_RS25285 (5264723) 5264723..5265625 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6036_RS25290 (5265622) 5265622..5266257 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6036_RS25295 (5266254) 5266254..5267183 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6036_RS25300 (5267357) 5267357..5267740 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6036_RS25305 (5267740) 5267740..5267982 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6036_RS25310 (5268187) 5268187..5269104 + 918 WP_004181612.1 alpha/beta hydrolase -
K6036_RS25315 (5269118) 5269118..5270059 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6036_RS25320 (5270104) 5270104..5270541 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6036_RS25325 (5270538) 5270538..5271398 - 861 WP_004146232.1 virulence factor BrkB family protein -
K6036_RS25330 (5271392) 5271392..5271991 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T214350 WP_002882817.1 NZ_CP082211:c5267740-5267357 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T214350 NZ_CP110050:c2647340-2647239 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT214350 WP_004150355.1 NZ_CP082211:c5267982-5267740 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT214350 NZ_CP110050:2647117-2647294 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCTTTTATACCAGCGCCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTTT
TTATTACTGTTTAACCGTTCGGCCTGTCAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACCGA
AAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References