Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4669539..4670349 Replicon chromosome
Accession NZ_CP082211
Organism Klebsiella pneumoniae strain A1718

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag K6036_RS22490 Protein ID WP_004178461.1
Coordinates 4669539..4670072 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K6036_RS22495 Protein ID WP_002887278.1
Coordinates 4670083..4670349 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6036_RS22485 (4668370) 4668370..4669491 + 1122 WP_002887282.1 cupin domain-containing protein -
K6036_RS22490 (4669539) 4669539..4670072 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
K6036_RS22495 (4670083) 4670083..4670349 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K6036_RS22500 (4670452) 4670452..4671885 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
K6036_RS22505 (4671875) 4671875..4672558 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K6036_RS22510 (4672730) 4672730..4674115 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
K6036_RS22515 (4674133) 4674133..4674477 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T214346 WP_004178461.1 NZ_CP082211:c4670072-4669539 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T214346 NZ_CP110050:388962-389270 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT214346 WP_002887278.1 NZ_CP082211:c4670349-4670083 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT214346 NZ_CP110050:388688-388960 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References