Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 44612..45194 Replicon plasmid pKPN43G-KPC
Accession NZ_CP082209
Organism Klebsiella pneumoniae strain KPN43G

Toxin (Protein)


Gene name PumA Uniprot ID E8PSG2
Locus tag K6032_RS27210 Protein ID WP_013583078.1
Coordinates 44898..45194 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A223LLE5
Locus tag K6032_RS27205 Protein ID WP_058998984.1
Coordinates 44612..44896 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6032_RS27170 (K6032_27135) 40124..40417 - 294 WP_058998996.1 TrbC/VirB2 family protein -
K6032_RS27175 (K6032_27140) 40435..41064 - 630 WP_058998994.1 lytic transglycosylase domain-containing protein -
K6032_RS27180 (K6032_27145) 41071..41268 - 198 WP_058998992.1 hypothetical protein -
K6032_RS27185 (K6032_27150) 41258..41752 - 495 WP_058998990.1 transcription termination/antitermination NusG family protein -
K6032_RS27190 (K6032_27155) 42144..42551 + 408 WP_072223386.1 helix-turn-helix transcriptional regulator -
K6032_RS27195 (K6032_27160) 42548..43717 - 1170 WP_058998988.1 MobP1 family relaxase -
K6032_RS27200 (K6032_27165) 43721..44308 - 588 WP_161400389.1 hypothetical protein -
K6032_RS27205 (K6032_27170) 44612..44896 - 285 WP_058998984.1 putative addiction module antidote protein Antitoxin
K6032_RS27210 (K6032_27175) 44898..45194 - 297 WP_013583078.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K6032_RS27215 (K6032_27180) 45533..45670 - 138 WP_161400388.1 hypothetical protein -
K6032_RS27220 (K6032_27185) 45717..45929 - 213 WP_058998981.1 hypothetical protein -
K6032_RS27225 (K6032_27190) 46335..46568 - 234 WP_072223385.1 hypothetical protein -
K6032_RS27230 (K6032_27195) 46558..46815 - 258 WP_058998978.1 hypothetical protein -
K6032_RS27235 (K6032_27200) 46812..47354 - 543 WP_058998977.1 hypothetical protein -
K6032_RS27240 (K6032_27205) 47354..47560 - 207 WP_058998975.1 hypothetical protein -
K6032_RS27245 (K6032_27210) 47572..47787 - 216 WP_058998973.1 hypothetical protein -
K6032_RS27250 (K6032_27215) 47829..48350 - 522 WP_058998971.1 J domain-containing protein -
K6032_RS27255 (K6032_27220) 48463..48684 + 222 WP_058998969.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..49053 49053


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(46-89)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11290.10 Da        Isoelectric Point: 10.3166

>T214330 WP_013583078.1 NZ_CP082209:c45194-44898 [Klebsiella pneumoniae]
MIEIKRTPEVEKWLRSLKDRTAKAKILMRLERMEDGNFGDVEPIGDGLSELRIHQGKGYRVYFGNKNNQIILLLCGGDKS
TQQADIRKAKQLAKQWGF

Download         Length: 297 bp

>T214330 NZ_CP110041:2815910-2816050 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10557.97 Da        Isoelectric Point: 4.7254

>AT214330 WP_058998984.1 NZ_CP082209:c44896-44612 [Klebsiella pneumoniae]
MKEELKKFDVVEFLEDEEDIQEYLNAAIEEDDTKYLFKALGNIARARNISQLSKEVGMSREGIYKALSGEGNPSFQTVSK
ITKALGLKLHFTGA

Download         Length: 285 bp

>AT214330 NZ_CP110041:c2816184-2815983 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A223LLP6

Antitoxin

Source ID Structure
AlphaFold DB A0A223LLE5

References