Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 174158..175068 Replicon plasmid pKPN43G-2FIB
Accession NZ_CP082208
Organism Klebsiella pneumoniae strain KPN43G

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag K6032_RS26765 Protein ID WP_004026354.1
Coordinates 174158..174628 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag K6032_RS26770 Protein ID WP_004026357.1
Coordinates 174625..175068 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6032_RS26745 (K6032_26715) 169829..170323 - 495 WP_004212794.1 thermonuclease family protein -
K6032_RS26750 170501..170767 + 267 WP_223175074.1 DUF1173 family protein -
K6032_RS26755 (K6032_26720) 170800..171450 + 651 WP_223356693.1 DUF1173 family protein -
K6032_RS26765 (K6032_26730) 174158..174628 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
K6032_RS26770 (K6032_26735) 174625..175068 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
K6032_RS26775 (K6032_26740) 175169..175672 - 504 Protein_180 DUF4113 domain-containing protein -
K6032_RS26780 (K6032_26745) 175770..175991 - 222 WP_004213643.1 DUF2188 domain-containing protein -
K6032_RS26785 (K6032_26750) 176166..176291 - 126 Protein_182 Fe3+-citrate ABC transporter substrate-binding protein -
K6032_RS26790 (K6032_26755) 176366..178492 - 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
K6032_RS26795 (K6032_26760) 178579..179532 - 954 Protein_184 ferric citrate uptake sigma factor regulator FecR -
K6032_RS26800 (K6032_26765) 179529..180050 - 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - pla / rmpA / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA 1..207355 207355


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T214329 WP_004026354.1 NZ_CP082208:c174628-174158 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T214329 NZ_CP110041:2815910-2816050 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT214329 WP_004026357.1 NZ_CP082208:c175068-174625 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT214329 NZ_CP110041:c2815908-2815765 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References