Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 135158..135894 Replicon plasmid pKPN43G-2FIB
Accession NZ_CP082208
Organism Klebsiella pneumoniae strain KPN43G

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag K6032_RS26565 Protein ID WP_004098919.1
Coordinates 135412..135894 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag K6032_RS26560 Protein ID WP_004213599.1
Coordinates 135158..135424 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6032_RS26540 (K6032_26510) 132671..133675 + 1005 WP_000427620.1 IS110-like element IS4321 family transposase -
K6032_RS26545 (K6032_26515) 133961..134455 + 495 WP_004213594.1 hypothetical protein -
K6032_RS26550 (K6032_26520) 134516..134719 + 204 WP_004213596.1 HHA domain-containing protein -
K6032_RS26555 (K6032_26525) 134733..134963 + 231 WP_004213598.1 hypothetical protein -
K6032_RS26560 (K6032_26530) 135158..135424 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
K6032_RS26565 (K6032_26535) 135412..135894 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
K6032_RS26570 (K6032_26540) 136315..137542 + 1228 Protein_138 IS3 family transposase -
K6032_RS26575 (K6032_26545) 137538..137933 - 396 Protein_139 IS3 family transposase -
K6032_RS26580 (K6032_26550) 138108..139130 - 1023 WP_004214536.1 porphobilinogen synthase -
K6032_RS26585 (K6032_26555) 139194..140540 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - pla / rmpA / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA 1..207355 207355
- inside IScluster/Tn - - 118304..137542 19238


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T214328 WP_004098919.1 NZ_CP082208:135412-135894 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T214328 NZ_CP110041:2815642-2815791 [Enterococcus faecalis]
ATGCGTATGCACGTATTTCCGAAATTTAGAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGG
GTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT214328 WP_004213599.1 NZ_CP082208:135158-135424 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT214328 NZ_CP110041:c2815908-2815724 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References