Detailed information of TA system TA214327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 66004..66674
• Replicon: plasmid pKPN43G-2FIB
• Accession: NZ_CP082208
• Organism: Klebsiella pneumoniae strain KPN43G

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: K6032_RS26210
• Protein ID: WP_004213072.1
• Coordinates: 66231..66674 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: K6032_RS26205
• Protein ID: WP_004213073.1
• Coordinates: 66004..66234 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6032_RS26180 (K6032_26150)	62227..63126	-	900	WP_004225022.1	nucleotide-binding protein	-
K6032_RS26185 (K6032_26155)	63116..63406	-	291	WP_004213078.1	hypothetical protein	-
K6032_RS26190 (K6032_26160)	63758..63964	+	207	WP_004213077.1	hypothetical protein	-
K6032_RS26195 (K6032_26165)	63954..64247	-	294	WP_004213076.1	hypothetical protein	-
K6032_RS26200 (K6032_26170)	64263..65396	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
K6032_RS26205 (K6032_26175)	66004..66234	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K6032_RS26210 (K6032_26180)	66231..66674	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6032_RS26215	66823..67074	+	252	WP_186987481.1	hypothetical protein	-
K6032_RS26220 (K6032_26190)	67097..67401	-	305	Protein_68	transposase	-
K6032_RS26225 (K6032_26195)	67818..68453	+	636	WP_165475474.1	mucoid phenotype regulator RmpA2	-
K6032_RS26230 (K6032_26200)	68971..69374	-	404	Protein_70	GAF domain-containing protein	-
K6032_RS26235 (K6032_26205)	69465..70385	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
K6032_RS26240 (K6032_26210)	70434..70925	-	492	WP_011251327.1	DM13 domain-containing protein	-
K6032_RS26245 (K6032_26215)	70988..71263	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pla / rmpA / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA	1..207355	207355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T214327 WP_004213072.1 NZ_CP082208:66231-66674 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T214327 NZ_CP110041:c2810202-2809861 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT214327 WP_004213073.1 NZ_CP082208:66004-66234 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT214327 NZ_CP110041:c2810432-2810202 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620