Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5291250..5291875 Replicon chromosome
Accession NZ_CP082207
Organism Klebsiella pneumoniae strain KPN43G

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6032_RS25515 Protein ID WP_002882817.1
Coordinates 5291250..5291633 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6032_RS25520 Protein ID WP_004150355.1
Coordinates 5291633..5291875 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6032_RS25500 (5288616) 5288616..5289518 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6032_RS25505 (5289515) 5289515..5290150 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6032_RS25510 (5290147) 5290147..5291076 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6032_RS25515 (5291250) 5291250..5291633 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6032_RS25520 (5291633) 5291633..5291875 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6032_RS25525 (5292080) 5292080..5292997 + 918 WP_004178029.1 alpha/beta hydrolase -
K6032_RS25530 (5293011) 5293011..5293952 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6032_RS25535 (5293997) 5293997..5294434 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6032_RS25540 (5294431) 5294431..5295291 - 861 WP_004146232.1 virulence factor BrkB family protein -
K6032_RS25545 (5295285) 5295285..5295884 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T214326 WP_002882817.1 NZ_CP082207:c5291633-5291250 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T214326 NZ_CP110041:c2665484-2665383 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT214326 WP_004150355.1 NZ_CP082207:c5291875-5291633 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT214326 NZ_CP110041:2665259-2665438 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGT
TTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACC
GAAAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References