Detailed information of TA system TA214325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4967206..4968887
• Replicon: chromosome
• Accession: NZ_CP082207
• Organism: Klebsiella pneumoniae strain KPN43G

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: K6032_RS24005
• Protein ID: WP_004146663.1
• Coordinates: 4967206..4968123 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: K6032_RS24010
• Protein ID: WP_004177809.1
• Coordinates: 4968129..4968887 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6032_RS23985 (4962512)	4962512..4964032	+	1521	WP_004210021.1	sugar ABC transporter ATP-binding protein	-
K6032_RS23990 (4964029)	4964029..4965027	+	999	WP_002885172.1	ribose ABC transporter permease	-
K6032_RS23995 (4965058)	4965058..4965999	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
K6032_RS24000 (4966074)	4966074..4967081	+	1008	WP_004177805.1	carbohydrate kinase family protein	-
K6032_RS24005 (4967206)	4967206..4968123	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
K6032_RS24010 (4968129)	4968129..4968887	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
K6032_RS24015 (4968973)	4968973..4969701	-	729	WP_004177810.1	response regulator transcription factor	-
K6032_RS24020 (4969974)	4969974..4972121	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
K6032_RS24025 (4972118)	4972118..4972261	-	144	WP_022631365.1	hypothetical protein	-
K6032_RS24030 (4972467)	4972467..4973153	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T214325 WP_004146663.1 NZ_CP082207:c4968123-4967206 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T214325 NZ_CP110041:c2665051-2664950 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT214325 WP_004177809.1 NZ_CP082207:c4968887-4968129 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT214325 NZ_CP110041:2664831-2665005 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTT
TTGAATCTTAACCGTTCGGCCTACGAAGCTTGTGAACGGTTATTTTTTATTGTTTTTCGTTGCTTCAAGGATACCGAAAA
TCAGTAGTGCAACAA

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05