Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4714669..4715479 Replicon chromosome
Accession NZ_CP082207
Organism Klebsiella pneumoniae strain KPN43G

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag K6032_RS22765 Protein ID WP_004178461.1
Coordinates 4714669..4715202 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K6032_RS22770 Protein ID WP_002887278.1
Coordinates 4715213..4715479 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6032_RS22760 (4713500) 4713500..4714621 + 1122 WP_020804439.1 cupin domain-containing protein -
K6032_RS22765 (4714669) 4714669..4715202 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
K6032_RS22770 (4715213) 4715213..4715479 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K6032_RS22775 (4715582) 4715582..4717015 - 1434 WP_020804438.1 Cu(+)/Ag(+) sensor histidine kinase -
K6032_RS22780 (4717005) 4717005..4717688 - 684 WP_020804437.1 copper response regulator transcription factor CusR -
K6032_RS22785 (4717860) 4717860..4719245 + 1386 WP_020804440.1 efflux transporter outer membrane subunit -
K6032_RS22790 (4719263) 4719263..4719607 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T214323 WP_004178461.1 NZ_CP082207:c4715202-4714669 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T214323 NZ_CP110041:433453-433761 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT214323 WP_002887278.1 NZ_CP082207:c4715479-4715213 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT214323 NZ_CP110041:433209-433451 [Enterococcus faecalis]
ATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAATTATGTTTTGAATCATGAACTAGATACTAA
AAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCACAAGAAGTTGATTTATTTCAACAACCATTTG
AAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCAAAACAAATTTTGACAAAAGAGAAAGTGGCT
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References