Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2754524..2756112 Replicon chromosome
Accession NZ_CP082207
Organism Klebsiella pneumoniae strain KPN43G

Toxin (Protein)


Gene name hipA Uniprot ID W9B834
Locus tag K6032_RS13415 Protein ID WP_016529318.1
Coordinates 2754524..2755846 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A483IVM3
Locus tag K6032_RS13420 Protein ID WP_029496892.1
Coordinates 2755846..2756112 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6032_RS13400 (2749580) 2749580..2752081 + 2502 WP_032410312.1 fimbria/pilus outer membrane usher protein -
K6032_RS13405 (2752069) 2752069..2753073 + 1005 WP_009485243.1 fimbrial protein -
K6032_RS13410 (2753215) 2753215..2754507 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
K6032_RS13415 (2754524) 2754524..2755846 - 1323 WP_016529318.1 type II toxin-antitoxin system HipA family toxin Toxin
K6032_RS13420 (2755846) 2755846..2756112 - 267 WP_029496892.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
K6032_RS13425 (2756499) 2756499..2756825 - 327 WP_002904788.1 hydroxyisourate hydrolase -
K6032_RS13430 (2756822) 2756822..2757322 - 501 WP_020804988.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
K6032_RS13435 (2757300) 2757300..2758679 - 1380 WP_094935985.1 nucleobase:cation symporter-2 family protein -
K6032_RS13440 (2759025) 2759025..2760179 + 1155 WP_020804972.1 FAD-dependent urate hydroxylase HpxO -
K6032_RS13445 (2760160) 2760160..2761083 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - mgtC / iroE 2562504..2791724 229220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-64)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49125.44 Da        Isoelectric Point: 7.5366

>T214321 WP_016529318.1 NZ_CP082207:c2755846-2754524 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T214321 NZ_CP110040:c2644870-2644529 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9938.33 Da        Isoelectric Point: 8.5912

>AT214321 WP_029496892.1 NZ_CP082207:c2756112-2755846 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGMVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT214321 NZ_CP110040:c2645100-2644870 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACATTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9B834

Antitoxin

Source ID Structure
AlphaFold DB A0A483IVM3

References