Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 325747..326333 Replicon chromosome
Accession NZ_CP082207
Organism Klebsiella pneumoniae strain KPN43G

Toxin (Protein)


Gene name doc Uniprot ID A0A486RB10
Locus tag K6032_RS01510 Protein ID WP_004174007.1
Coordinates 325965..326333 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag K6032_RS01505 Protein ID WP_004174006.1
Coordinates 325747..325968 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6032_RS01485 (321904) 321904..322830 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
K6032_RS01490 (322827) 322827..324104 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
K6032_RS01495 (324101) 324101..324868 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
K6032_RS01500 (324870) 324870..325583 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
K6032_RS01505 (325747) 325747..325968 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K6032_RS01510 (325965) 325965..326333 + 369 WP_004174007.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K6032_RS01515 (326606) 326606..327922 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
K6032_RS01520 (328029) 328029..328916 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
K6032_RS01525 (328913) 328913..329758 + 846 WP_004174009.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
K6032_RS01530 (329760) 329760..330830 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 315516..331567 16051


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(6-116)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13568.97 Da        Isoelectric Point: 8.6410

>T214310 WP_004174007.1 NZ_CP082207:325965-326333 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDLGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T214310 NZ_CP110036:202989-203090 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT214310 WP_004174006.1 NZ_CP082207:325747-325968 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT214310 NZ_CP110036:c203214-203035 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGT
TTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACC
GAAAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486RB10

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References