Detailed information of TA system TA214306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-couple_hipB
• Location: 1413248..1414491
• Replicon: chromosome
• Accession: NZ_CP082206
• Organism: Streptococcus dysgalactiae strain DY107

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: G7SEB4
• Locus tag: K6973_RS07235
• Protein ID: WP_001235242.1
• Coordinates: 1413248..1414015 (-)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: sezA
• Uniprot ID: -
• Locus tag: K6973_RS07240
• Protein ID: WP_000578719.1
• Coordinates: 1414015..1414491 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6973_RS07195 (K6973_07195)	1408585..1409394	-	810	WP_002294509.1	ANT(9) family aminoglycoside nucleotidyltransferase Spw	-
K6973_RS07200 (K6973_07200)	1409526..1410053	-	528	WP_002294507.1	phosphoribosyltransferase family protein	-
K6973_RS07205 (K6973_07205)	1410097..1410960	-	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
K6973_RS07210 (K6973_07210)	1410979..1411095	-	117	Protein_1384	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
K6973_RS07215 (K6973_07215)	1411265..1412002	-	738	WP_002292226.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
K6973_RS07220 (K6973_07220)	1412127..1412210	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
K6973_RS07225 (K6973_07225)	1412259..1412498	-	240	WP_000635249.1	peptide-binding protein	-
K6973_RS07230 (K6973_07230)	1412618..1413169	-	552	Protein_1388	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
K6973_RS07235 (K6973_07235)	1413248..1414015	-	768	WP_001235242.1	type II toxin-antitoxin system toxin PezT	Toxin
K6973_RS07240 (K6973_07240)	1414015..1414491	-	477	WP_000578719.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
K6973_RS07245 (K6973_07245)	1414561..1414851	-	291	WP_000667951.1	hypothetical protein	-
K6973_RS07250 (K6973_07250)	1414902..1415291	-	390	WP_222819187.1	DUF5945 family protein	-
K6973_RS07255 (K6973_07255)	1415288..1415515	-	228	WP_023370180.1	DUF5965 family protein	-
K6973_RS07260 (K6973_07260)	1415569..1416645	-	1077	WP_222819188.1	toprim domain-containing protein	-
K6973_RS07265 (K6973_07265)	1416708..1418564	-	1857	WP_001820670.1	recombinase family protein	-
K6973_RS07270 (K6973_07270)	1418619..1418882	-	264	WP_000253176.1	hypothetical protein	-
K6973_RS07275 (K6973_07275)	1418882..1419112	-	231	WP_001206986.1	PC4/YdbC family ssDNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	cat / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'') / lnu(B) / lsa(E) / erm(B) / tet(O)	-	1390174..1459073	68899
	-	flank	IS/Tn	cat / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'') / lnu(B) / lsa(E) / erm(B)	-	1396551..1412002	15451

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 28985.80 Da
• Isoelectric Point: 5.1126

>T214306 WP_001235242.1 NZ_CP082206:c1414015-1413248 [Streptococcus dysgalactiae]
MRLEEFSEAEFQKALQRTIRALTRGKTIPDQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEQLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLSSKGYQVSLAVIGTKPELSYLSTLIRYEE
LYAIDPTQARATPKEHHDGIVENLVDNLRELESENLFDQIQIYQRDRTCIYDSETAEGSAAEVLQECLFGKWSKVEEEMV
KESELLLKELLEKNK

Nucleotide

• Length: 768 bp

>T214306 NZ_CP110036:c52184-52011 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAAATAACCGTCTACTTTGACCAGT
ACGGTTATTTTTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18266.77 Da
• Isoelectric Point: 4.6424

>AT214306 WP_000578719.1 NZ_CP082206:c1414491-1414015 [Streptococcus dysgalactiae]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLTPVEDYHLTLKVE
VIKERGAAILSKLYRYQDSQDIVFDDESNPWILMSDDLAELINTKIYLVATFDEIERYNGYLDGIERMLDMVHHRVVA

Nucleotide

• Length: 477 bp

>AT214306 NZ_CP110036:52186-52329 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAA

Structures

Toxin

• AlphaFold DB: G7SEB4