Detailed information of TA system TA214304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 1243190..1244298
• Replicon: chromosome
• Accession: NZ_CP082203
• Organism: Streptococcus suis strain NJ3

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: K6972_RS06175
• Protein ID: WP_000233000.1
• Coordinates: 1243190..1244059 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: K6972_RS06180
• Protein ID: WP_000205227.1
• Coordinates: 1244074..1244298 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6972_RS06155 (K6972_06155)	1240129..1241010	-	882	WP_222760822.1	hypothetical protein	-
K6972_RS06160 (K6972_06160)	1241089..1241571	-	483	Protein_1173	IS6 family transposase	-
K6972_RS06165 (K6972_06165)	1241579..1242442	-	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
K6972_RS06170 (K6972_06170)	1242475..1243209	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
K6972_RS06175 (K6972_06175)	1243190..1244059	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
K6972_RS06180 (K6972_06180)	1244074..1244298	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
K6972_RS06185 (K6972_06185)	1244441..1244526	-	86	Protein_1178	site-specific recombinase	-
K6972_RS06190 (K6972_06190)	1244751..1244903	+	153	Protein_1179	imsC-like protein	-
K6972_RS06195 (K6972_06195)	1244916..1245284	+	369	WP_222760820.1	hypothetical protein	-
K6972_RS06200 (K6972_06200)	1245271..1245570	+	300	WP_172055574.1	DUF5960 family protein	-
K6972_RS06205 (K6972_06205)	1245756..1247219	-	1464	WP_015057857.1	ABC-F type ribosomal protection protein Msr(D)	-
K6972_RS06210 (K6972_06210)	1247340..1248557	-	1218	WP_222760818.1	macrolide efflux MFS transporter Mef(A)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IS/Tn	ant(6)-Ia / msr(D) / mef(A)	-	1241089..1248557	7468

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T214304 WP_000233000.1 NZ_CP082203:c1244059-1243190 [Streptococcus suis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T214304 NZ_CP110036:c51812-51669 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGAAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT214304 WP_000205227.1 NZ_CP082203:c1244298-1244074 [Streptococcus suis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT214304 NZ_CP110036:51909-52111 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTTCTTGTCATTTTTAAGC
AATTTCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: R3JIN1