Detailed information of TA system TA214277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 597252..598360
• Replicon: chromosome
• Accession: NZ_CP082198
• Organism: Streptococcus suis strain Transconjugant cNJ3

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: K6968_RS02970
• Protein ID: WP_000233000.1
• Coordinates: 597491..598360 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: K6968_RS02965
• Protein ID: WP_000205227.1
• Coordinates: 597252..597476 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6968_RS02935 (K6968_02935)	592993..594210	+	1218	WP_222760818.1	macrolide efflux MFS transporter Mef(A)	-
K6968_RS02940 (K6968_02940)	594331..595794	+	1464	WP_015057857.1	ABC-F type ribosomal protection protein Msr(D)	-
K6968_RS02945 (K6968_02945)	595980..596279	-	300	WP_172055574.1	DUF5960 family protein	-
K6968_RS02950 (K6968_02950)	596266..596634	-	369	WP_222760820.1	hypothetical protein	-
K6968_RS02955 (K6968_02955)	596647..596799	-	153	Protein_535	imsC-like protein	-
K6968_RS02960 (K6968_02960)	597024..597109	+	86	Protein_536	site-specific recombinase	-
K6968_RS02965 (K6968_02965)	597252..597476	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
K6968_RS02970 (K6968_02970)	597491..598360	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
K6968_RS02975 (K6968_02975)	598341..599075	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
K6968_RS02980 (K6968_02980)	599108..599971	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
K6968_RS02985 (K6968_02985)	599979..600461	+	483	Protein_541	IS6 family transposase	-
K6968_RS02990 (K6968_02990)	600540..601421	+	882	WP_222760822.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IS/Tn	mef(A) / msr(D) / ant(6)-Ia	-	592993..600461	7468

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T214277 WP_000233000.1 NZ_CP082198:597491-598360 [Streptococcus suis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T214277 NZ_CP110018:c1501347-1501153 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT214277 WP_000205227.1 NZ_CP082198:597252-597476 [Streptococcus suis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT214277 NZ_CP110018:1500976-1501154 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: R3JIN1