Detailed information of TA system TA214271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 17092..17645
• Replicon: plasmid unnamed1
• Accession: NZ_CP082179
• Organism: Xanthomonas fragariae strain SHQP01

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K4A87_RS19830
• Protein ID: WP_208591899.1
• Coordinates: 17352..17645 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K4A87_RS19825
• Protein ID: WP_208591900.1
• Coordinates: 17092..17364 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4A87_RS19795 (K4A87_19650)	12542..13074	-	533	Protein_17	transposase	-
K4A87_RS19800 (K4A87_19655)	13277..13645	-	369	WP_208591874.1	hypothetical protein	-
K4A87_RS19805 (K4A87_19660)	14161..14463	-	303	WP_208591907.1	hypothetical protein	-
K4A87_RS19810 (K4A87_19665)	14460..14750	-	291	WP_208591905.1	hypothetical protein	-
K4A87_RS19815 (K4A87_19670)	15036..15344	+	309	WP_208591903.1	MobB mobilization protein	-
K4A87_RS19820 (K4A87_19675)	15341..17017	+	1677	WP_208591901.1	relaxase/mobilization nuclease domain-containing protein	-
K4A87_RS19825 (K4A87_19680)	17092..17364	+	273	WP_208591900.1	CopG family ribbon-helix-helix protein	Antitoxin
K4A87_RS19830 (K4A87_19685)	17352..17645	+	294	WP_208591899.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K4A87_RS19835 (K4A87_19690)	17776..17970	+	195	WP_208591909.1	hypothetical protein	-
K4A87_RS19840 (K4A87_19695)	18000..18353	-	354	WP_208591898.1	hypothetical protein	-
K4A87_RS19845 (K4A87_19700)	18362..18676	-	315	WP_238135756.1	hypothetical protein	-
K4A87_RS19850 (K4A87_19705)	18636..19145	-	510	WP_208591896.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..20157	20157
	-	flank	IS/Tn	-	-	12754..13074	320

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-39)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10877.36 Da
• Isoelectric Point: 5.6404

>T214271 WP_208591899.1 NZ_CP082179:17352-17645 [Xanthomonas fragariae]
VPHVIITEGAGQGLECCRRFLASKSPEAARRAAKAIERQFLLLETTPAIGRPFSEQPEWSELVIEFGDSGYVALYRHEAA
EDVVYVLAFRHQKEVGY

Nucleotide

• Length: 294 bp

>T214271 NZ_CP110018:226405-226803 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10452.62 Da
• Isoelectric Point: 5.5812

>AT214271 WP_208591900.1 NZ_CP082179:17092-17364 [Xanthomonas fragariae]
MATSLKIDDELKGRVQHLASLRDRSPHWIMREAIKQYVELEEARESFKQEAQASWIAYQETGRHLTGQEARAWLNTWGTD
NETGLPECHT

Nucleotide

• Length: 273 bp

>AT214271 NZ_CP110018:226109-226402 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA