Detailed information of TA system TA214268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1535356..1536071
• Replicon: chromosome
• Accession: NZ_CP082178
• Organism: Xanthomonas fragariae strain SHQP01

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K4A87_RS07175
• Protein ID: WP_208590583.1
• Coordinates: 1535652..1536071 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K4A87_RS07170
• Protein ID: WP_238135661.1
• Coordinates: 1535356..1535655 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4A87_RS07155 (K4A87_07145)	1533027..1533560	+	534	Protein_1332	transposase	-
K4A87_RS07160 (K4A87_07150)	1533644..1534138	-	495	WP_208590579.1	hypothetical protein	-
K4A87_RS07165 (K4A87_07155)	1534708..1534989	+	282	WP_002805735.1	TraK family protein	-
K4A87_RS07170 (K4A87_07160)	1535356..1535655	+	300	WP_238135661.1	Arc family DNA-binding protein	Antitoxin
K4A87_RS07175 (K4A87_07165)	1535652..1536071	+	420	WP_208590583.1	type II toxin-antitoxin system VapC family toxin	Toxin
K4A87_RS07180	1536404..1536511	+	108	WP_238135662.1	helix-turn-helix domain-containing protein	-
K4A87_RS07185	1536750..1536845	+	96	Protein_1338	IS30 family transposase	-
K4A87_RS07190 (K4A87_07175)	1536995..1537225	+	231	Protein_1339	transposase	-
K4A87_RS07195 (K4A87_07180)	1537290..1538261	+	972	WP_208590244.1	IS5 family transposase	-
K4A87_RS07200 (K4A87_07185)	1538390..1538970	+	581	Protein_1341	IS5/IS1182 family transposase	-
K4A87_RS07205 (K4A87_07190)	1539063..1539437	-	375	WP_208590590.1	Mth938-like domain-containing protein	-
K4A87_RS07210 (K4A87_07195)	1539454..1539759	-	306	WP_208590591.1	ribosome assembly RNA-binding protein YhbY	-
K4A87_RS07215 (K4A87_07200)	1539826..1540458	+	633	WP_208590599.1	23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	1529399..1538919	9520

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (24-61)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14881.06 Da
• Isoelectric Point: 4.6936

>T214268 WP_208590583.1 NZ_CP082178:1535652-1536071 [Xanthomonas fragariae]
MIVLDTNVVSEAMKPEPNPAVRAWLNEQVAETLYLSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFSERVLMFDTE
AARNYAELAVKARTAGKGFPTPDGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNNQ

Nucleotide

• Length: 420 bp

>T214268 NZ_CP110017:4549191-4549541 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11205.77 Da
• Isoelectric Point: 4.7746

>AT214268 WP_238135661.1 NZ_CP082178:1535356-1535655 [Xanthomonas fragariae]
MCNIEVNEVTYEVLTMAILTVRNVPDEVHRALRLRAAEHGRSTEAEVREILESAVKSEKRVRMGDALAELGRQVGLTNDD
FAVLDQVRDKVPAEPMRFE

Nucleotide

• Length: 300 bp

>AT214268 NZ_CP110017:4548946-4549197 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA