Detailed information of TA system TA214262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii(toxin)
• Location: 9136561..9137795
• Replicon: chromosome
• Accession: NZ_CP082164
• Organism: Bradyrhizobium sp. 186

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: IVB18_RS43485
• Protein ID: WP_247986220.1
• Coordinates: 9136875..9137795 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: IVB18_RS43480
• Protein ID: WP_247986219.1
• Coordinates: 9136561..9136875 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IVB18_RS43470 (IVB18_42895)	9132113..9136171	-	4059	WP_256477132.1	helix-turn-helix transcriptional regulator	-
IVB18_RS43475	9136174..9136527	-	354	WP_247986218.1	hypothetical protein	-
IVB18_RS43480	9136561..9136875	+	315	WP_247986219.1	hypothetical protein	Antitoxin
IVB18_RS43485 (IVB18_42905)	9136875..9137795	+	921	WP_247986220.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
IVB18_RS43490 (IVB18_42915)	9138674..9140134	-	1461	WP_247986221.1	HEPN domain-containing protein	-
IVB18_RS43495 (IVB18_42920)	9140178..9140471	+	294	WP_247986222.1	hypothetical protein	-
IVB18_RS43505 (IVB18_42930)	9141002..9141436	-	435	WP_247986223.1	phasin	-
IVB18_RS43510 (IVB18_42935)	9141716..9142162	-	447	WP_247986224.1	phasin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-272)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34235.63 Da
• Isoelectric Point: 5.6592

>T214262 WP_247986220.1 NZ_CP082164:9136875-9137795 [Bradyrhizobium sp. 186]
MAKVLKNLAASIRDRLLQHTKQHGDVYQRILTRYAIERLLFRLSLTEAAEHYVLKGAMLFVTWPEHVFRPTGDLDLLGHG
DPEPAAIVELFTRICQVEAPDDGIIFDPATLTVEPVREGDKYQGVHLNLKGELARAVIPVQVDIGFGDHVYPPPERRMFP
CLLPNLPAANILMYPPETVIAEKFEVMIRFGTANGRIKDFYDIWVTLRTFGFELPELVEAVGGTLRRRETAVPTEIPVGL
TEAFATIAADRGLWTGFLRRNLPTIQPPSFPELQLELQRFLGPVITGLAVPEGAKGRWDPDGGAWV

Nucleotide

• Length: 921 bp

>T214262 NZ_CP110017:c3134059-3133652 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11680.56 Da
• Isoelectric Point: 9.5973

>AT214262 WP_247986219.1 NZ_CP082164:9136561-9136875 [Bradyrhizobium sp. 186]
MTIPHKARTPNAQVMRLEIVRATGDVLTAGVEHVRVEGVDVPIYGVAKTIADCFKHRRHVGEDVAIEALRDALRQRKTTE
SELMKYAGIDRVTARIEPYIKAMQ

Nucleotide

• Length: 315 bp

>AT214262 NZ_CP110017:c3134306-3134040 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA