Detailed information of TA system TA214255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 1109554..1110287
• Replicon: chromosome
• Accession: NZ_CP082164
• Organism: Bradyrhizobium sp. 186

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: IVB18_RS05010
• Protein ID: WP_247988165.1
• Coordinates: 1109889..1110287 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: IVB18_RS05005
• Protein ID: WP_247988164.1
• Coordinates: 1109554..1109892 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IVB18_RS04980 (IVB18_04950)	1105245..1105565	+	321	WP_247988159.1	hypothetical protein	-
IVB18_RS04985 (IVB18_04955)	1105693..1106097	+	405	WP_247988160.1	hypothetical protein	-
IVB18_RS04990 (IVB18_04960)	1106428..1107711	-	1284	WP_247988161.1	site-specific integrase	-
IVB18_RS04995 (IVB18_04965)	1108032..1108589	+	558	WP_247988162.1	hypothetical protein	-
IVB18_RS05000 (IVB18_04970)	1108604..1109371	+	768	WP_247988163.1	hypothetical protein	-
IVB18_RS05005 (IVB18_04975)	1109554..1109892	-	339	WP_247988164.1	helix-turn-helix transcriptional regulator	Antitoxin
IVB18_RS05010 (IVB18_04980)	1109889..1110287	-	399	WP_247988165.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IVB18_RS05015 (IVB18_04985)	1110744..1110965	-	222	WP_247988166.1	hypothetical protein	-
IVB18_RS05020 (IVB18_04990)	1110960..1111235	+	276	WP_247991556.1	DUF2384 domain-containing protein	-
IVB18_RS05025 (IVB18_04995)	1112019..1112195	+	177	WP_247988167.1	hypothetical protein	-
IVB18_RS05030 (IVB18_05000)	1112841..1113089	-	249	WP_247988168.1	AEC family transporter	-
IVB18_RS05035 (IVB18_05005)	1113141..1113572	-	432	WP_247988169.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1099814..1116548	16734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-90)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14945.31 Da
• Isoelectric Point: 10.6004

>T214255 WP_247988165.1 NZ_CP082164:c1110287-1109889 [Bradyrhizobium sp. 186]
MKEVTIKRPEPKPCVFIGSSRKDLKRFPAKVQNRMGHALHQVQEGDEPIAAKALKGFGGRAILELIDDFDSDTYRAVYTV
RFAGVVYVLHAFQKKAKKGIATPQRDIELIKSRLRDAEEHYRARADKGGRKP

Nucleotide

• Length: 399 bp

>T214255 NZ_CP110017:c2011791-2011688 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12624.63 Da
• Isoelectric Point: 8.5310

>AT214255 WP_247988164.1 NZ_CP082164:c1109892-1109554 [Bradyrhizobium sp. 186]
MTKHKIEAYEGSGNIFADLGLPNPEERLLKSSIVAELHRLIRERGLTQVKAAKLIGVSQPDLSHLLRGDYDDYSAERLMK
MLTVFEQDIEIVMRPHRKAGEPGRITFKPVAA

Nucleotide

• Length: 339 bp

>AT214255 NZ_CP110017:2011650-2011921 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT