Detailed information of TA system TA214254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 78859..80389
• Replicon: plasmid p195_2
• Accession: NZ_CP082163
• Organism: Bradyrhizobium sp. 195

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: IVB26_RS42130
• Protein ID: WP_247974081.1
• Coordinates: 79469..80389 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: IVB26_RS42125
• Protein ID: WP_247974080.1
• Coordinates: 78859..79479 (+)
• Length: 207 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IVB26_RS42110 (IVB26_41750)	75263..75490	+	228	WP_247974077.1	hypothetical protein	-
IVB26_RS42115 (IVB26_41755)	75483..77966	+	2484	WP_247974078.1	AAA family ATPase	-
IVB26_RS42120 (IVB26_41760)	77966..78685	+	720	WP_247974079.1	DCL family protein	-
IVB26_RS42125 (IVB26_41765)	78859..79479	+	621	WP_247974080.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
IVB26_RS42130 (IVB26_41770)	79469..80389	+	921	WP_247974081.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
IVB26_RS42135 (IVB26_41775)	80467..80637	+	171	WP_247974082.1	hypothetical protein	-
IVB26_RS42140 (IVB26_41780)	80634..81866	+	1233	WP_247974083.1	exonuclease SbcCD subunit D	-
IVB26_RS42145	81869..83803	+	1935	WP_247974084.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..220947	220947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-267)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (15-56)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 33785.85 Da
• Isoelectric Point: 4.8780

>T214254 WP_247974081.1 NZ_CP082163:79469-80389 [Bradyrhizobium sp. 195]
MAREPRNIGASVRARLLDRARAERSDFQILLTRYALERLLYRLSVSPHRDRFILKGAMLFVTWVADPFRPTRDLDLLGNG
DSDAEAIAETFRAICAQPVADDGVTFDIAALMAAPIREEVEYGGVRVRTTATIAGARIPIQVDIGFGDAITPAAVEIDYP
TLLDAPTPHLRAYPVETVVAEKFEALVTLGVANSRLKDFYDLWVISRTFELRRAALVEAIQRTFERRGTVLPSVVPVGLT
DEFAEAWAAQWRAFLGRDRMAAAPAAFAIAVADLRLFLMPLVVGLEEERIWPPSGPWSPGAAIDEA

Nucleotide

• Length: 921 bp

>T214254 NZ_CP110017:c1733273-1732986 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 207 a.a.
• Molecular weight: 23359.37 Da
• Isoelectric Point: 10.3406

>AT214254 WP_247974080.1 NZ_CP082163:78859-79479 [Bradyrhizobium sp. 195]
MPSASVQRLRAIDLLKTRGMLRLKDFVAEGIGPETLARLVREEVVVRPARGLYQLPDAHVEAAHTLAEAAVLVPKGVVCL
TSALQYHELTLQMPSAVWMAIDRVAWRPKIEYPAIRFVRFTGSALTEGVERHRIENVDVPITNPAKTIVDCFRYRTKIGL
DVAMEGLREGLRRRRCKPDQLWRYARKARVWSVMRPYVEAMVSDGA

Nucleotide

• Length: 621 bp

>AT214254 NZ_CP110017:c1733512-1733273 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA