Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 12433..12998 Replicon plasmid pRop44186_b
Accession NZ_CP082158
Organism Rhodococcus opacus strain DSM 44186

Toxin (Protein)


Gene name mazF Uniprot ID A0A149ZUW0
Locus tag FXW36_RS00080 Protein ID WP_061043268.1
Coordinates 12433..12789 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID X0Q3L2
Locus tag FXW36_RS00085 Protein ID WP_037232310.1
Coordinates 12786..12998 (-) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FXW36_RS00055 (FXW36_00055) 8037..8744 - 708 WP_061043275.1 hypothetical protein -
FXW36_RS00060 (FXW36_00060) 9242..9622 + 381 Protein_12 AAA family ATPase -
FXW36_RS00065 (FXW36_00065) 9869..10186 + 318 WP_120659064.1 type II toxin-antitoxin system RelE/ParE family toxin -
FXW36_RS00070 (FXW36_00070) 10183..10488 + 306 WP_061043273.1 XRE family transcriptional regulator -
FXW36_RS00075 (FXW36_00075) 11199..11393 - 195 WP_061043271.1 hypothetical protein -
FXW36_RS00080 (FXW36_00080) 12433..12789 - 357 WP_061043268.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FXW36_RS00085 (FXW36_00085) 12786..12998 - 213 WP_037232310.1 ribbon-helix-helix domain-containing protein Antitoxin
FXW36_RS00090 (FXW36_00090) 13446..14222 - 777 WP_120659065.1 VOC family protein -
FXW36_RS00095 (FXW36_00095) 15147..16337 - 1191 WP_120659067.1 Fic family protein -
FXW36_RS00100 (FXW36_00100) 16776..17195 - 420 WP_167120112.1 HNH endonuclease -
FXW36_RS00105 (FXW36_00105) 17365..17721 - 357 WP_133986696.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..52207 52207


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-114)

Antitoxin

Ribbon-helix-helix protein, CopG

(4-40)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12831.58 Da        Isoelectric Point: 7.3223

>T214251 WP_061043268.1 NZ_CP082158:c12789-12433 [Rhodococcus opacus]
MIRRGQVHWVDLDPAQGSEAAKRRPAVVVSNDRANATAERLGQGVVTVVPLTSNTAHVFAFQVLVEPDESGMPRESKAQA
EQVRSVSVRRLDGDPVGKLSTRTLAALEEALRLHLDLL

Download         Length: 357 bp

>T214251 NZ_CP110017:c1501339-1501145 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 7516.23 Da        Isoelectric Point: 4.0552

>AT214251 WP_037232310.1 NZ_CP082158:c12998-12786 [Rhodococcus opacus]
MTTRFTISLPDEDARVLDAYVRDHGLGGRSAGIRAAVRMLGEVGLERDYAAAFVEGGVDDDWDVTSADGE

Download         Length: 213 bp

>AT214251 NZ_CP110017:1500968-1501146 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A149ZUW0

Antitoxin

Source ID Structure
AlphaFold DB X0Q3L2

References