Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 479314..479932 Replicon chromosome
Accession NC_007779
Organism Escherichia coli str. K-12 substr. W3110

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag Y75_RS02365 Protein ID WP_001291435.1
Coordinates 479314..479532 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag Y75_RS02370 Protein ID WP_000344800.1
Coordinates 479558..479932 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y75_RS02330 (Y75_p0441) 474603..475175 + 573 WP_000779842.1 YbaY family lipoprotein -
Y75_RS02335 (Y75_p0442) 475206..475517 - 312 WP_000409911.1 MGMT family protein -
Y75_RS02345 (Y75_p0443) 475896..476249 + 354 WP_000878140.1 DUF1428 domain-containing protein -
Y75_RS02350 (Y75_p0444) 476291..477841 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
Y75_RS02355 (Y75_p0445) 478005..478475 - 471 WP_000136192.1 YlaC family protein -
Y75_RS02360 (Y75_p0446) 478591..479142 - 552 WP_000102564.1 maltose O-acetyltransferase -
Y75_RS02365 (Y75_p0447) 479314..479532 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
Y75_RS02370 (Y75_p0448) 479558..479932 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
Y75_RS02375 (Y75_p0449) 480478..483627 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
Y75_RS02380 (Y75_p0450) 483650..484843 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T21425 WP_001291435.1 NC_007779:c479532-479314 [Escherichia coli str. K-12 substr. W3110]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T21425 NC_007779:c479532-479314 [Escherichia coli str. K-12 substr. W3110]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT21425 WP_000344800.1 NC_007779:c479932-479558 [Escherichia coli str. K-12 substr. W3110]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT21425 NC_007779:c479932-479558 [Escherichia coli str. K-12 substr. W3110]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References