Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 13650..14293 Replicon plasmid p66R
Accession NZ_CP082156
Organism Klebsiella pneumoniae strain Kp11-81

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag K6977_RS27435 Protein ID WP_000754566.1
Coordinates 13877..14293 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag K6977_RS27430 Protein ID WP_001261276.1
Coordinates 13650..13880 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6977_RS27395 (K6977_27325) 8769..9038 + 270 WP_000339857.1 hypothetical protein -
K6977_RS27400 (K6977_27330) 9215..10081 - 867 WP_004118283.1 replication initiation protein -
K6977_RS27405 (K6977_27335) 10611..10715 + 105 WP_032409716.1 hypothetical protein -
K6977_RS27410 (K6977_27340) 10844..11101 + 258 WP_000764642.1 hypothetical protein -
K6977_RS27415 (K6977_27345) 11159..11935 - 777 WP_032446863.1 site-specific integrase -
K6977_RS27420 (K6977_27350) 11932..12675 - 744 WP_000129823.1 hypothetical protein -
K6977_RS27425 (K6977_27355) 12726..13076 - 351 WP_000493378.1 hypothetical protein -
K6977_RS27430 (K6977_27360) 13650..13880 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6977_RS27435 (K6977_27365) 13877..14293 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
K6977_RS27440 (K6977_27370) 14497..15353 + 857 Protein_16 IS3-like element ISEc15 family transposase -
K6977_RS27445 (K6977_27375) 15635..15835 + 201 Protein_17 transposase -
K6977_RS27450 (K6977_27380) 15858..16280 + 423 WP_032446862.1 hypothetical protein -
K6977_RS27455 (K6977_27385) 16289..16786 + 498 WP_032446861.1 hypothetical protein -
K6977_RS27460 (K6977_27390) 16923..17258 - 336 Protein_20 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aac(6')-Ib-cr / blaOXA-1 / qnrB1 / aac(3)-IIa / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 - 1..66722 66722


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T214249 WP_000754566.1 NZ_CP082156:13877-14293 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T214249 NZ_CP110017:c1309269-1309162 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT214249 WP_001261276.1 NZ_CP082156:13650-13880 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT214249 NZ_CP110017:1309316-1309382 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References