Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 124193..124929 Replicon plasmid p220F
Accession NZ_CP082155
Organism Klebsiella pneumoniae strain Kp11-81

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag K6977_RS26860 Protein ID WP_003026803.1
Coordinates 124447..124929 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag K6977_RS26855 Protein ID WP_003026799.1
Coordinates 124193..124459 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6977_RS26810 (K6977_26750) 120255..120617 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
K6977_RS26815 (K6977_26755) 120667..121017 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
K6977_RS26820 (K6977_26760) 121375..121644 + 270 WP_004152102.1 hypothetical protein -
K6977_RS26825 (K6977_26765) 121632..122207 + 576 WP_004152103.1 hypothetical protein -
K6977_RS26830 (K6977_26770) 122238..122732 + 495 WP_004152104.1 DNA-binding protein -
K6977_RS26835 (K6977_26775) 122776..123144 + 369 WP_004152105.1 hypothetical protein -
K6977_RS26840 (K6977_26780) 123178..123381 + 204 WP_004152106.1 HHA domain-containing protein -
K6977_RS26845 (K6977_26785) 123430..123687 + 258 WP_004152107.1 hypothetical protein -
K6977_RS26850 (K6977_26790) 123763..124017 + 255 WP_004152108.1 hypothetical protein -
K6977_RS26855 (K6977_26795) 124193..124459 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
K6977_RS26860 (K6977_26800) 124447..124929 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
K6977_RS26865 (K6977_26805) 125141..126487 + 1347 WP_020314316.1 ISNCY family transposase -
K6977_RS28155 126648..126779 + 132 WP_004218042.1 hypothetical protein -
K6977_RS26870 (K6977_26810) 126988..128153 - 1166 Protein_138 IS3 family transposase -
K6977_RS26875 (K6977_26815) 128330..129292 - 963 WP_004152113.1 zinc metalloprotease HtpX -
K6977_RS26880 (K6977_26820) 129279..129767 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1 - 1..220228 220228


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T214248 WP_003026803.1 NZ_CP082155:124447-124929 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T214248 NZ_CP110017:c1308734-1308627 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT214248 WP_003026799.1 NZ_CP082155:124193-124459 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT214248 NZ_CP110017:1308782-1308848 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References