Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1839610..1840235 Replicon chromosome
Accession NZ_CP082154
Organism Klebsiella pneumoniae strain Kp11-81

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6977_RS08930 Protein ID WP_002882817.1
Coordinates 1839852..1840235 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6977_RS08925 Protein ID WP_004150355.1
Coordinates 1839610..1839852 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6977_RS08900 (K6977_08890) 1835601..1836200 + 600 WP_004151865.1 glucose-1-phosphatase -
K6977_RS08905 (K6977_08895) 1836194..1837054 + 861 WP_002882807.1 virulence factor BrkB family protein -
K6977_RS08910 (K6977_08900) 1837051..1837488 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6977_RS08915 (K6977_08905) 1837533..1838474 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6977_RS08920 (K6977_08910) 1838488..1839405 - 918 WP_002882812.1 alpha/beta hydrolase -
K6977_RS08925 (K6977_08915) 1839610..1839852 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6977_RS08930 (K6977_08920) 1839852..1840235 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6977_RS08935 (K6977_08925) 1840409..1841338 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6977_RS08940 (K6977_08930) 1841335..1841970 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6977_RS08945 (K6977_08935) 1841967..1842869 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T214234 WP_002882817.1 NZ_CP082154:1839852-1840235 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T214234 NZ_CP110016:2868581-2868910 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT214234 WP_004150355.1 NZ_CP082154:1839610-1839852 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT214234 NZ_CP110016:2868243-2868560 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References