Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 16828..17471 Replicon plasmid unnamed3
Accession NZ_CP082150
Organism Enterobacter cloacae complex sp. strain ECL42

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag K6966_RS26800 Protein ID WP_000754567.1
Coordinates 17055..17471 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A155IUD1
Locus tag K6966_RS26795 Protein ID WP_023293777.1
Coordinates 16828..17058 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6966_RS27230 11830..11901 + 72 Protein_12 hypothetical protein -
K6966_RS27235 12037..12693 + 657 WP_226856284.1 hypothetical protein -
K6966_RS26775 (K6966_26775) 12625..13491 - 867 WP_023302476.1 replication initiation protein -
K6966_RS26780 (K6966_26780) 14132..14389 + 258 WP_000764642.1 hypothetical protein -
K6966_RS26785 (K6966_26785) 14447..15235 - 789 WP_022631000.1 site-specific integrase -
K6966_RS26790 (K6966_26790) 15255..15926 - 672 WP_024140048.1 hypothetical protein -
K6966_RS26795 (K6966_26795) 16828..17058 + 231 WP_023293777.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6966_RS26800 (K6966_26800) 17055..17471 + 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
K6966_RS26805 (K6966_26805) 17822..18790 - 969 WP_163467572.1 IS5 family transposase -
K6966_RS26810 (K6966_26810) 18950..19213 + 264 WP_001295708.1 hypothetical protein -
K6966_RS26815 (K6966_26815) 19407..19688 + 282 WP_023332908.1 helix-turn-helix domain-containing protein -
K6966_RS26820 (K6966_26820) 19723..20292 + 570 WP_023332907.1 small heat shock protein sHSP20 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..37659 37659
- flank IS/Tn - - 17822..18790 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T214229 WP_000754567.1 NZ_CP082150:17055-17471 [Enterobacter cloacae complex sp.]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T214229 NZ_CP110016:c2011788-2011685 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8706.73 Da        Isoelectric Point: 4.4299

>AT214229 WP_023293777.1 NZ_CP082150:16828-17058 [Enterobacter cloacae complex sp.]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Download         Length: 231 bp

>AT214229 NZ_CP110016:2011647-2011918 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A155IUD1

References