Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 27786..29409 Replicon plasmid unnamed1
Accession NZ_CP082148
Organism Enterobacter cloacae complex sp. strain ECL42

Toxin (Protein)


Gene name hipA Uniprot ID A0A7G9A813
Locus tag K6966_RS23735 Protein ID WP_020833823.1
Coordinates 27786..29111 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A411GQR5
Locus tag K6966_RS23740 Protein ID WP_024143029.1
Coordinates 29116..29409 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6966_RS23705 (K6966_23705) 23773..24630 - 858 WP_020833820.1 HNH endonuclease signature motif containing protein -
K6966_RS23710 (K6966_23710) 24646..24954 - 309 WP_001224686.1 hypothetical protein -
K6966_RS23715 (K6966_23715) 25502..25927 - 426 WP_020833821.1 hypothetical protein -
K6966_RS23720 (K6966_23720) 26179..26994 - 816 WP_024143028.1 HNH endonuclease signature motif containing protein -
K6966_RS23725 (K6966_23725) 27007..27417 - 411 WP_000985911.1 hypothetical protein -
K6966_RS23730 (K6966_23730) 27519..27725 - 207 WP_000134171.1 hypothetical protein -
K6966_RS23735 (K6966_23735) 27786..29111 - 1326 WP_020833823.1 type II toxin-antitoxin system HipA family toxin Toxin
K6966_RS23740 (K6966_23740) 29116..29409 - 294 WP_024143029.1 helix-turn-helix transcriptional regulator Antitoxin
K6966_RS23745 (K6966_23745) 29840..31228 - 1389 WP_008786875.1 hypothetical protein -
K6966_RS23750 (K6966_23750) 31233..31730 - 498 WP_008786874.1 membrane protein -
K6966_RS23755 (K6966_23755) 31932..32579 - 648 WP_008786873.1 hypothetical protein -
K6966_RS23760 (K6966_23760) 33535..33927 - 393 WP_000341066.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - vipB/tssC / vipA/tssB 1..338256 338256


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(155-404)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-70)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50230.15 Da        Isoelectric Point: 6.1424

>T214227 WP_020833823.1 NZ_CP082148:c29111-27786 [Enterobacter cloacae complex sp.]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPEWSADEQGRPLSLSIPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAEHDKAQFFMAQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T214227 NZ_CP110016:c1679817-1678495 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10846.21 Da        Isoelectric Point: 10.3134

>AT214227 WP_024143029.1 NZ_CP082148:c29409-29116 [Enterobacter cloacae complex sp.]
INNEYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSVLGVKISFSSTATSSDSK
QTEETLKSNSPARQEKW

Download         Length: 294 bp

>AT214227 NZ_CP110016:c1680083-1679817 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G9A813

Antitoxin

Source ID Structure
AlphaFold DB A0A411GQR5

References