Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3035881..3036471 Replicon chromosome
Accession NZ_CP082147
Organism Enterobacter cloacae complex sp. strain ECL42

Toxin (Protein)


Gene name pemK Uniprot ID A0A8B2Y1G2
Locus tag K6966_RS14515 Protein ID WP_045134957.1
Coordinates 3035881..3036213 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag K6966_RS14520 Protein ID WP_058690818.1
Coordinates 3036214..3036471 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6966_RS14500 (K6966_14500) 3032045..3033370 + 1326 WP_058690820.1 SidA/IucD/PvdA family monooxygenase -
K6966_RS14505 (K6966_14505) 3033397..3035586 + 2190 WP_222834586.1 TonB-dependent siderophore receptor -
K6966_RS14515 (K6966_14515) 3035881..3036213 - 333 WP_045134957.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K6966_RS14520 (K6966_14520) 3036214..3036471 - 258 WP_058690818.1 hypothetical protein Antitoxin
K6966_RS14525 (K6966_14525) 3036837..3037027 - 191 Protein_2856 helix-turn-helix transcriptional regulator -
K6966_RS14530 (K6966_14530) 3037315..3038433 - 1119 WP_023337501.1 beta-ketoacyl synthase N-terminal-like domain-containing protein -
K6966_RS14535 (K6966_14535) 3038490..3039173 - 684 WP_014884440.1 hypothetical protein -
K6966_RS14540 (K6966_14540) 3039183..3040628 - 1446 WP_078310457.1 class I adenylate-forming enzyme family protein -
K6966_RS14545 (K6966_14545) 3040631..3040894 - 264 WP_078310462.1 acyl carrier protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11785.63 Da        Isoelectric Point: 10.5834

>T214223 WP_045134957.1 NZ_CP082147:c3036213-3035881 [Enterobacter cloacae complex sp.]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLDGAGTKTMGAIRCDQPRTI
DMAARSGKRLERIPDAVVNEVLARLDAILN

Download         Length: 333 bp

>T214223 NZ_CP110016:c1309272-1309165 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9455.96 Da        Isoelectric Point: 4.4488

>AT214223 WP_058690818.1 NZ_CP082147:c3036471-3036214 [Enterobacter cloacae complex sp.]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPVV
GKEIL

Download         Length: 258 bp

>AT214223 NZ_CP110016:1309319-1309385 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2Y1G2

Antitoxin

Source ID Structure

References