Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1141637..1142257 Replicon chromosome
Accession NZ_CP082147
Organism Enterobacter cloacae complex sp. strain ECL42

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag K6966_RS05430 Protein ID WP_014882802.1
Coordinates 1141637..1141855 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag K6966_RS05435 Protein ID WP_008499288.1
Coordinates 1141883..1142257 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6966_RS05400 (K6966_05400) 1137646..1137906 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
K6966_RS05405 (K6966_05405) 1137909..1138049 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
K6966_RS05410 (K6966_05410) 1138046..1138756 - 711 WP_222834794.1 GNAT family protein -
K6966_RS05415 (K6966_05415) 1138858..1140318 + 1461 WP_058690566.1 PLP-dependent aminotransferase family protein -
K6966_RS05420 (K6966_05420) 1140290..1140757 - 468 WP_014882800.1 YlaC family protein -
K6966_RS05425 (K6966_05425) 1140876..1141427 - 552 WP_014882801.1 maltose O-acetyltransferase -
K6966_RS05430 (K6966_05430) 1141637..1141855 - 219 WP_014882802.1 HHA domain-containing protein Toxin
K6966_RS05435 (K6966_05435) 1141883..1142257 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
K6966_RS05440 (K6966_05440) 1142882..1146028 - 3147 WP_014882803.1 multidrug efflux RND transporter permease subunit AcrB -
K6966_RS05445 (K6966_05445) 1146051..1147244 - 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T214213 WP_014882802.1 NZ_CP082147:c1141855-1141637 [Enterobacter cloacae complex sp.]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T214213 NZ_CP110015:3374449-3374913 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT214213 WP_008499288.1 NZ_CP082147:c1142257-1141883 [Enterobacter cloacae complex sp.]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT214213 NZ_CP110015:3374114-3374449 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References