Detailed information of TA system TA214212

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 974142..974821
• Replicon: chromosome
• Accession: NZ_CP082147
• Organism: Enterobacter cloacae complex sp. strain ECL42

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: K6966_RS04695
• Protein ID: WP_016246873.1
• Coordinates: 974142..974483 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: K6966_RS04700
• Protein ID: WP_113372912.1
• Coordinates: 974504..974821 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6966_RS04670 (K6966_04670)	969332..969733	+	402	WP_014882682.1	sigma factor-binding protein Crl	-
K6966_RS04675 (K6966_04675)	969847..970899	-	1053	WP_014882683.1	phosphoporin PhoE	-
K6966_RS04680 (K6966_04680)	971205..972308	+	1104	WP_014882684.1	glutamate 5-kinase	-
K6966_RS04685 (K6966_04685)	972320..973573	+	1254	WP_045281951.1	glutamate-5-semialdehyde dehydrogenase	-
K6966_RS04695 (K6966_04695)	974142..974483	-	342	WP_016246873.1	TA system toxin CbtA family protein	Toxin
K6966_RS04700 (K6966_04700)	974504..974821	-	318	WP_113372912.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K6966_RS04705 (K6966_04705)	974840..975061	-	222	WP_063841895.1	DUF987 domain-containing protein	-
K6966_RS04710 (K6966_04710)	975070..975546	-	477	WP_000811693.1	RadC family protein	-
K6966_RS04715 (K6966_04715)	975562..976020	-	459	WP_113372854.1	antirestriction protein	-
K6966_RS04720 (K6966_04720)	976124..976363	-	240	WP_007869725.1	DUF905 domain-containing protein	-
K6966_RS04725 (K6966_04725)	976387..977208	-	822	WP_113372767.1	DUF932 domain-containing protein	-
K6966_RS04730 (K6966_04730)	977297..978160	-	864	WP_113372766.1	GTPase family protein	-
K6966_RS04735 (K6966_04735)	978282..979043	-	762	WP_127790460.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12987.05 Da
• Isoelectric Point: 10.2764

>T214212 WP_016246873.1 NZ_CP082147:c974483-974142 [Enterobacter cloacae complex sp.]
MKTLPATTQRAAKPCLAPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T214212 NZ_CP110015:c3331567-3331253 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11847.42 Da
• Isoelectric Point: 6.7339

>AT214212 WP_113372912.1 NZ_CP082147:c974821-974504 [Enterobacter cloacae complex sp.]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDVECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT214212 NZ_CP110015:c3331256-3330840 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA