Detailed information of TA system TA214211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 583590..584117
• Replicon: chromosome
• Accession: NZ_CP082147
• Organism: Enterobacter cloacae complex sp. strain ECL42

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K6966_RS02865
• Protein ID: WP_222834775.1
• Coordinates: 583842..584117 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A941UEC5
• Locus tag: K6966_RS02860
• Protein ID: WP_000535213.1
• Coordinates: 583590..583838 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6966_RS02830 (K6966_02830)	579678..580355	+	678	WP_222834771.1	hypothetical protein	-
K6966_RS02835 (K6966_02835)	580455..581273	+	819	WP_222834772.1	DUF932 domain-containing protein	-
K6966_RS02840 (K6966_02840)	581344..581817	+	474	WP_171402892.1	DNA repair protein RadC	-
K6966_RS02845 (K6966_02845)	581862..582188	+	327	WP_222834849.1	type IV toxin-antitoxin system YeeU family antitoxin	-
K6966_RS02850 (K6966_02850)	582224..582535	+	312	WP_222834773.1	TA system toxin CbtA family protein	-
K6966_RS02855 (K6966_02855)	582680..583513	+	834	WP_222834774.1	DUF4942 domain-containing protein	-
K6966_RS02860 (K6966_02860)	583590..583838	+	249	WP_000535213.1	ribbon-helix-helix domain-containing protein	Antitoxin
K6966_RS02865 (K6966_02865)	583842..584117	+	276	WP_222834775.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6966_RS02870 (K6966_02870)	584236..585024	+	789	WP_222834776.1	helix-turn-helix domain-containing protein	-
K6966_RS02875 (K6966_02875)	585245..585730	+	486	Protein_558	integrase	-
K6966_RS02880 (K6966_02880)	585839..586492	-	654	Protein_559	HNH endonuclease	-
K6966_RS02885 (K6966_02885)	586793..587248	+	456	WP_075210127.1	SymE family type I addiction module toxin	-
K6966_RS02890 (K6966_02890)	587292..588293	-	1002	WP_048995936.1	tryptophan--tRNA ligase	-
K6966_RS02895 (K6966_02895)	588494..589045	-	552	WP_127341142.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	fosA	-	554680..591922	37242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-42)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11008.36 Da
• Isoelectric Point: 4.8836

>T214211 WP_222834775.1 NZ_CP082147:583842-584117 [Enterobacter cloacae complex sp.]
MEVYWTLKAQDDLERIYRFALQYSRQHADDVLDRLIIGCAGLTAHPAIGIQQTRYEPHEVRKVLFDDYEVHYELRDNDIY
IVDLWHTKEER

Nucleotide

• Length: 276 bp

>T214211 NZ_CP110015:c3265655-3265359 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGAACATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9004.31 Da
• Isoelectric Point: 7.2042

>AT214211 WP_000535213.1 NZ_CP082147:583590-583838 [Enterobacter cloacae complex sp.]
MGSLTKQTISAQIPVELAAAVENLAIELDRSKSWIIKEALTSMLAERERRHQSIQAGLADVDAGRVVSHSDMVDFANRLK
KS

Nucleotide

• Length: 249 bp

>AT214211 NZ_CP110015:c3265357-3264962 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA