Detailed information of TA system TA214210

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 581862..582535
• Replicon: chromosome
• Accession: NZ_CP082147
• Organism: Enterobacter cloacae complex sp. strain ECL42

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: K6966_RS02850
• Protein ID: WP_222834773.1
• Coordinates: 582224..582535 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: K6966_RS02845
• Protein ID: WP_222834849.1
• Coordinates: 581862..582188 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6966_RS02825 (K6966_02825)	578494..579387	+	894	WP_222834770.1	50S ribosome-binding GTPase	-
K6966_RS02830 (K6966_02830)	579678..580355	+	678	WP_222834771.1	hypothetical protein	-
K6966_RS02835 (K6966_02835)	580455..581273	+	819	WP_222834772.1	DUF932 domain-containing protein	-
K6966_RS02840 (K6966_02840)	581344..581817	+	474	WP_171402892.1	DNA repair protein RadC	-
K6966_RS02845 (K6966_02845)	581862..582188	+	327	WP_222834849.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K6966_RS02850 (K6966_02850)	582224..582535	+	312	WP_222834773.1	TA system toxin CbtA family protein	Toxin
K6966_RS02855 (K6966_02855)	582680..583513	+	834	WP_222834774.1	DUF4942 domain-containing protein	-
K6966_RS02860 (K6966_02860)	583590..583838	+	249	WP_000535213.1	ribbon-helix-helix domain-containing protein	-
K6966_RS02865 (K6966_02865)	583842..584117	+	276	WP_222834775.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K6966_RS02870 (K6966_02870)	584236..585024	+	789	WP_222834776.1	helix-turn-helix domain-containing protein	-
K6966_RS02875 (K6966_02875)	585245..585730	+	486	Protein_558	integrase	-
K6966_RS02880 (K6966_02880)	585839..586492	-	654	Protein_559	HNH endonuclease	-
K6966_RS02885 (K6966_02885)	586793..587248	+	456	WP_075210127.1	SymE family type I addiction module toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	fosA	-	554680..591922	37242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-103)

Antitoxin

• Antitoxin CbeA (8-107)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11709.47 Da
• Isoelectric Point: 7.1327

>T214210 WP_222834773.1 NZ_CP082147:582224-582535 [Enterobacter cloacae complex sp.]
MKTLPASTQRAAKPCPTPVVVWQTLLTRLLEQHYGLTLNDTPFCDETVIQEHINAGITLADAVNFLVEKYELVRIDRRGF
SWQEQSPYLRAVDILRARQATGL

Nucleotide

• Length: 312 bp

>T214210 NZ_CP110015:c3134057-3133650 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12408.13 Da
• Isoelectric Point: 5.5564

>AT214210 WP_222834849.1 NZ_CP082147:581862-582188 [Enterobacter cloacae complex sp.]
MEKNDPIEWGLRRDIMPRVGARLVQEGNQLHYLVDRAGVTGIFNEAESRKLNAAFPHFVEQMELMLLSGELNPRYAHCVT
LYHDGFTCEADTLGSYGYVYIVIYPTQR

Nucleotide

• Length: 327 bp

>AT214210 NZ_CP110015:c3134304-3134038 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA