Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 123041..123666 Replicon plasmid pIncFIA
Accession NZ_CP082130
Organism Escherichia coli strain NDM_11.16372

Toxin (Protein)


Gene name vapC Uniprot ID A0A1D7QA53
Locus tag K5P77_RS24230 Protein ID WP_039023147.1
Coordinates 123041..123439 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D7QA08
Locus tag K5P77_RS24235 Protein ID WP_039023146.1
Coordinates 123439..123666 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P77_RS24215 (119276) 119276..119773 + 498 WP_000605862.1 entry exclusion protein -
K5P77_RS24220 (119805) 119805..120536 + 732 WP_000850422.1 conjugal transfer complement resistance protein TraT -
K5P77_RS24225 (120789) 120789..123032 + 2244 WP_094907434.1 type IV conjugative transfer system coupling protein TraD -
K5P77_RS24230 (123041) 123041..123439 - 399 WP_039023147.1 type II toxin-antitoxin system VapC family toxin Toxin
K5P77_RS24235 (123439) 123439..123666 - 228 WP_039023146.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A) - 1..136961 136961


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14885.26 Da        Isoelectric Point: 8.2824

>T214204 WP_039023147.1 NZ_CP082130:c123439-123041 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAAIHT
GQIRAELARQGRPVGPFDQMIAGHARCRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T214204 NZ_CP110015:2166582-2166956 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8549.62 Da        Isoelectric Point: 4.7513

>AT214204 WP_039023146.1 NZ_CP082130:c123666-123439 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAIGRTRLITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT214204 NZ_CP110015:2166125-2166493 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7QA53

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7QA08

References