Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 59338..59981 Replicon plasmid pIncFIA
Accession NZ_CP082130
Organism Escherichia coli strain NDM_11.16372

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag K5P77_RS23860 Protein ID WP_001034044.1
Coordinates 59338..59754 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag K5P77_RS23865 Protein ID WP_001261286.1
Coordinates 59751..59981 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P77_RS23835 (54591) 54591..54704 - 114 Protein_61 IS6 family transposase -
K5P77_RS23840 (54904) 54904..55601 + 698 WP_223350503.1 IS1 family transposase -
K5P77_RS23845 (55751) 55751..56437 + 687 Protein_63 IS1 family transposase -
K5P77_RS23850 (56691) 56691..57713 - 1023 WP_000361402.1 helicase UvrD -
K5P77_RS23855 (57698) 57698..59263 - 1566 WP_001128474.1 AAA family ATPase -
K5P77_RS23860 (59338) 59338..59754 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
K5P77_RS23865 (59751) 59751..59981 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K5P77_RS23870 (60541) 60541..60759 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
K5P77_RS23875 (60761) 60761..61066 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
K5P77_RS23880 (61067) 61067..61873 + 807 WP_000016970.1 site-specific integrase -
K5P77_RS23885 (62595) 62595..63350 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A) - 1..136961 136961


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T214199 WP_001034044.1 NZ_CP082130:c59754-59338 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T214199 NZ_CP110015:c1501339-1501145 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT214199 WP_001261286.1 NZ_CP082130:c59981-59751 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT214199 NZ_CP110015:1500968-1501146 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References