Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4385975..4386472 Replicon chromosome
Accession NZ_CP082129
Organism Escherichia coli strain NDM_11.16372

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K5P77_RS21205 Protein ID WP_001173343.1
Coordinates 4385975..4386148 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag K5P77_RS21210 Protein ID WP_000398619.1
Coordinates 4386176..4386472 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P77_RS21195 (4382645) 4382645..4384102 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
K5P77_RS21200 (4384339) 4384339..4385856 + 1518 WP_001295074.1 lysine--tRNA ligase -
K5P77_RS21205 (4385975) 4385975..4386148 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K5P77_RS21210 (4386176) 4386176..4386472 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K5P77_RS21215 (4386699) 4386699..4386971 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
K5P77_RS21220 (4386983) 4386983..4387213 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
K5P77_RS21225 (4387394) 4387394..4389025 + 1632 WP_001216477.1 sensor histidine kinase -
K5P77_RS21230 (4389022) 4389022..4389741 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T214198 WP_001173343.1 NZ_CP082129:c4386148-4385975 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T214198 NZ_CP110015:c1309805-1309698 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT214198 WP_000398619.1 NZ_CP082129:c4386472-4386176 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT214198 NZ_CP110015:1309852-1309918 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References