Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4168378..4168954 Replicon chromosome
Accession NZ_CP082129
Organism Escherichia coli strain NDM_11.16372

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag K5P77_RS20085 Protein ID WP_001297643.1
Coordinates 4168667..4168954 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag K5P77_RS20080 Protein ID WP_000063160.1
Coordinates 4168378..4168680 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P77_RS20065 (4164974) 4164974..4167124 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
K5P77_RS20070 (4167174) 4167174..4167377 + 204 WP_000467859.1 YbdD/YjiX family protein -
K5P77_RS20075 (4167388) 4167388..4168344 + 957 WP_001297640.1 GTPase -
K5P77_RS20080 (4168378) 4168378..4168680 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
K5P77_RS20085 (4168667) 4168667..4168954 - 288 WP_001297643.1 BrnT family toxin Toxin
K5P77_RS20090 (4169153) 4169153..4169849 - 697 Protein_3951 IS1 family transposase -
K5P77_RS20095 (4170164) 4170164..4171396 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
K5P77_RS20100 (4171437) 4171437..4172717 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4169153..4169362 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T214196 WP_001297643.1 NZ_CP082129:c4168954-4168667 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T214196 NZ_CP110015:c1308735-1308628 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT214196 WP_000063160.1 NZ_CP082129:c4168680-4168378 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT214196 NZ_CP110015:1308783-1308849 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References