Detailed information of TA system TA214192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2503724..2504362
• Replicon: chromosome
• Accession: NZ_CP082129
• Organism: Escherichia coli strain NDM_11.16372

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: K5P77_RS12020
• Protein ID: WP_001447010.1
• Coordinates: 2504186..2504362 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K5P77_RS12015
• Protein ID: WP_001270286.1
• Coordinates: 2503724..2504140 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P77_RS11995 (2498876)	2498876..2499817	-	942	WP_001251304.1	ABC transporter permease	-
K5P77_RS12000 (2499818)	2499818..2500831	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
K5P77_RS12005 (2500849)	2500849..2501994	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
K5P77_RS12010 (2502239)	2502239..2503645	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
K5P77_RS12015 (2503724)	2503724..2504140	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K5P77_RS12020 (2504186)	2504186..2504362	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K5P77_RS12025 (2504584)	2504584..2504814	+	231	WP_000494244.1	YncJ family protein	-
K5P77_RS12030 (2504906)	2504906..2506867	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K5P77_RS12035 (2506940)	2506940..2507476	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
K5P77_RS12040 (2507568)	2507568..2508743	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T214192 WP_001447010.1 NZ_CP082129:c2504362-2504186 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T214192 NZ_CP110015:c220345-220067 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT214192 WP_001270286.1 NZ_CP082129:c2504140-2503724 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT214192 NZ_CP110015:c220608-220348 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8