Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 60541..61066 Replicon plasmid pIncFIA
Accession NZ_CP082128
Organism Escherichia coli strain NDM_12_14482

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag K5P76_RS23870 Protein ID WP_001159871.1
Coordinates 60761..61066 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag K5P76_RS23865 Protein ID WP_000813630.1
Coordinates 60541..60759 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P76_RS23840 (55751) 55751..56437 + 687 Protein_63 IS1 family transposase -
K5P76_RS23845 (56691) 56691..57713 - 1023 WP_000361402.1 helicase UvrD -
K5P76_RS23850 (57698) 57698..59263 - 1566 WP_001128474.1 AAA family ATPase -
K5P76_RS23855 (59338) 59338..59754 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
K5P76_RS23860 (59751) 59751..59981 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
K5P76_RS23865 (60541) 60541..60759 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
K5P76_RS23870 (60761) 60761..61066 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
K5P76_RS23875 (61067) 61067..61873 + 807 WP_000016970.1 site-specific integrase -
K5P76_RS23880 (62595) 62595..63350 + 756 WP_000852146.1 replication initiation protein RepE -
K5P76_RS23885 (63938) 63938..65104 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A) - 1..136961 136961


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T214169 WP_001159871.1 NZ_CP082128:60761-61066 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T214169 NZ_CP110014:c418863-418645 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT214169 WP_000813630.1 NZ_CP082128:60541-60759 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT214169 NZ_CP110014:c419263-418889 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References