Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4168266..4168842 Replicon chromosome
Accession NZ_CP082127
Organism Escherichia coli strain NDM_12_14482

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag K5P76_RS20080 Protein ID WP_001297643.1
Coordinates 4168555..4168842 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag K5P76_RS20075 Protein ID WP_000063160.1
Coordinates 4168266..4168568 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P76_RS20060 (4164862) 4164862..4167012 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
K5P76_RS20065 (4167062) 4167062..4167265 + 204 WP_000467859.1 YbdD/YjiX family protein -
K5P76_RS20070 (4167276) 4167276..4168232 + 957 WP_001297640.1 GTPase -
K5P76_RS20075 (4168266) 4168266..4168568 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
K5P76_RS20080 (4168555) 4168555..4168842 - 288 WP_001297643.1 BrnT family toxin Toxin
K5P76_RS20085 (4169041) 4169041..4169737 - 697 Protein_3951 IS1 family transposase -
K5P76_RS20090 (4170052) 4170052..4171284 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
K5P76_RS20095 (4171325) 4171325..4172605 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4169041..4169250 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T214165 WP_001297643.1 NZ_CP082127:c4168842-4168555 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T214165 NZ_CP110006:c2726477-2726184 [Flavobacterium sp. N2469]
ATGGCTAAATATCATTTTACAAATAAAGCTGTTGAAGATTTAGCTGATATTTGGAATTATACTTTTGATGAATGGTCAGA
AAATCAAGCTGATAAATATTACCTTTTGCTTTTAGATTCTTGTCAGGAATTGGCAGAGAATCCAAATCTTGGAAAAAAAT
ATGATACTGTCGCAGAAAGTTTATTTGGGTTTAAATCAAACCTGCATATTCTTTTTTATCAAATTATTTCAAACACCGAA
ATTGAAGTTGTTAGGATTCTTCATGGAAGAATGGATTTAAAAAGTAAGTTTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT214165 WP_000063160.1 NZ_CP082127:c4168568-4168266 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT214165 NZ_CP110006:c2726718-2726470 [Flavobacterium sp. N2469]
ATGGGAAGAAATACATCAATATCGCTTGGAAATCATTTTGAAAACTTCATTGAAAATAGTCTTTCTGAGGGAAGGTTTAA
GAATGCAAGTGAAGTTGTTAGAGCAGGATTACGTCTTTTAGAAGAAGAAGAGAATAAATTACTTATTTTGAAAAAAGCCG
TTCAGGAAGGACTTGAAAGTGGACGTGCTGAGAACTTTGATTCCAAAAAACATCTTGAAAGTTTAAAAGCAAATAGAAAA
CATGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References