Detailed information of TA system TA214161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2503613..2504251
• Replicon: chromosome
• Accession: NZ_CP082127
• Organism: Escherichia coli strain NDM_12_14482

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: K5P76_RS12015
• Protein ID: WP_001447010.1
• Coordinates: 2504075..2504251 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K5P76_RS12010
• Protein ID: WP_001270286.1
• Coordinates: 2503613..2504029 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P76_RS11990 (2498765)	2498765..2499706	-	942	WP_001251304.1	ABC transporter permease	-
K5P76_RS11995 (2499707)	2499707..2500720	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
K5P76_RS12000 (2500738)	2500738..2501883	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
K5P76_RS12005 (2502128)	2502128..2503534	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
K5P76_RS12010 (2503613)	2503613..2504029	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K5P76_RS12015 (2504075)	2504075..2504251	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K5P76_RS12020 (2504473)	2504473..2504703	+	231	WP_000494244.1	YncJ family protein	-
K5P76_RS12025 (2504795)	2504795..2506756	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K5P76_RS12030 (2506829)	2506829..2507365	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
K5P76_RS12035 (2507457)	2507457..2508632	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T214161 WP_001447010.1 NZ_CP082127:c2504251-2504075 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T214161 NZ_CP109989:705-1019 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT214161 WP_001270286.1 NZ_CP082127:c2504029-2503613 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT214161 NZ_CP109989:439-717 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8